콘텐츠로 건너뛰기
Merck

NA0200

GenElute™ HP Plasmid Midiprep Kit

greener alternative

sufficient for 25 purifications

동의어(들):

GenElute™ HP Plasmid Midiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.52
UNSPSC Code:
41105501

주요 문서

모든 문서 보기
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의

usage

sufficient for 25 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

Quality Level

200

test parameters

sample volume: 1-5 mL bacterial culture

greener alternative category

, Aligned

storage temp.

15-25°C

관련 카테고리

General description

The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Click here to view the kit protocol

Application

GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.

Features and Benefits

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 350 μg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • No phenol/chloroform extraction or alcohol precipitation required
  • Contains fewer plastic components, reducing the amount of waste

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • R1149Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )SDS
  • W0263Wash Solution 1SDS
  • W4639Wash Solution 2SDS
  • R6148RNase A solutionSDS
  • C2112Column Preparation SolutionSDS
  • L1912Lysis SolutionSDS

signalword

Danger

target_organs

Central nervous system

저장 등급

3 - Flammable liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000476568
0000476568
0000385570
0000333635
0000385570

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Steinhart Z
Nature Medicine, 23(1), 60-68 (2017)
Vanessa E Gray et al.
G3 (Bethesda, Md.), 9(11), 3683-3689 (2019-09-29)
Despite the importance of Aβ aggregation in Alzheimer's disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of Aβ40 proposed core
The distal upstream promoter in Ly49 genes, Pro1, is active in mature NK cells and T cells, does not require TATA boxes, and displays enhancer activity.
Gays F
Journal of Immunology, 194(12), 6068-6081 (2015)
Stephanie P Cartwright et al.
BMC research notes, 10(1), 340-340 (2017-07-29)
Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV
Theresa B Loveless et al.
Nature chemical biology, 17(6), 739-747 (2021-03-24)
Studying cellular and developmental processes in complex multicellular organisms can require the non-destructive observation of thousands to billions of cells deep within an animal. DNA recorders address the staggering difficulty of this task by converting transient cellular experiences into mutations
모든 관련된 서류 보기

관련 콘텐츠

mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

mRNA 합성

mRNA 기반 백신 및 치료제 개발에서 플라스미드 준비, 시험관 내 전사, mRNA 정제 및 LNP 제형을 위한 도구입니다.

DNA 및 RNA 정제

세포, 조직, 혈액, 바이러스 및 기타 시료 유형으로부터 유전체 DNA, 플리스미드 DNA 및 총 RNA의 추출과 정제를 위한 일반적인 기법과 다운스트림 애플리케이션의 개요.

모두 보기

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.