P0372
Anti-Podocin antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
동의어(들):
Podocin Antibody, Podocin Antibody - Anti-Podocin antibody produced in rabbit
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
polyclonal
Application:
indirect immunofluorescence
western blot (chemiluminescent)
western blot (chemiluminescent)
Species reactivity:
human, rat, mouse
Citations:
145
Technique(s):
indirect immunofluorescence: 10-20 μg/mL using acetone-fixed human or rat kidney frozen sections
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli
Uniprot accession no.:
제품 이름
Anti-Podocin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa (doublet)
species reactivity
human, rat, mouse
technique(s)
indirect immunofluorescence: 10-20 μg/mL using acetone-fixed human or rat kidney frozen sections
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NPHS2(7827)
mouse ... Nphs2(170484)
rat ... Nphs2(170672)
Application
Anti-Podocin antibody produced in rabbit has been used for Western Blotting and immunohistochemistry.
Biochem/physiol Actions
Anti-Podocin recognizes human, mouse, and rat podocin.
Podocin and nephrin are essential for maintaining the slit diaphragm. Studies show that mutations in this gene account for around 15% cases of nephrotic syndrome cases. Podocin interacts with cholesterol and promotes the formation of protein-lipid supercomplexes, which control ion channel complexes. The podocin gene is known to be associated with autosomal recessive SRNS (nephrotic syndrome type 2). Podocin actively participates in the ultrafiltration process occuring in kidney.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Podocin is a podocyte structural protein, that belongs to the band-7-stomatin family of lipid raft-associated proteins. This family contains more than 1800 conserved proteins which share ∼150 amino acid domains showing similarity to mitochondrial protein prohibitin (PHB).
Podocin is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, an intercellular junction found in mammalian kidney.
Podocin is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, an intercellular junction found in mammalian kidney.
Immunogen
Synthetic peptide corresponding to amino acid residues of human podocin with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Excess podocyte semaphorin-3A leads to glomerular disease involving plexinA1-nephrin interaction.
Reidy KJ et al
The American Journal of Pathology, 183(4), 1156-1168 (2013)
NPHS2 mutations account for only 15% of nephrotic syndrome cases.
Guaragna MS et al
BMC Medical Genetics, 16(88) (2015)
Batoul Wehbi et al.
Journal of the American Society of Nephrology : JASN, 30(7), 1238-1249 (2019-06-23)
IgA nephropathy (IgAN) often follows infections and features IgA mesangial deposition. Polymeric IgA deposits in the mesangium seem to have varied pathogenic potential, but understanding their pathogenicity remains a challenge. Most mesangial IgA1 in human IgAN has a hypogalactosylated hinge
The SPFH domain-containing proteins: more than lipid raft markers.
Browman D T, et al.
Trends in Cell Biology, 17(8), 394-402 (2007)
Induction of podocyte VEGF164 overexpression at different stages of development causes congenital nephrosis or steroid-resistant nephrotic syndrome.
Veron D et al
The American Journal of Pathology, 177(5), 2225-2233 (2010)
관련 콘텐츠
Datasheet
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