P3391

Protein A-Sepharose^™ from Staphylococcus aureus

Name: Protein A-Sepharose<SUP>™</SUP> from <I>Staphylococcus aureus</I>
Brand: SIGMA

lyophilized powder

동의어(들):

Protein A–Agarose

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

41106500

NACRES:

NA.56

MDL number:

MFCD00165563

Form:

lyophilized powder

주요 문서

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기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

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도움 문의

form

lyophilized powder

packaging

glass bottle of 1 g, glass bottle of 1.5 g, glass bottle of 250 mg, glass bottle of 5 g

storage condition

do not freeze

extent of labeling

3 mg per mL

technique(s)

affinity chromatography: suitable

color

white to gray

matrix

Sepharose CL-4B

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥16 mg/mL binding capacity (human IgG)

solubility

water: 1 g/L at 20 °C

suitability

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

storage temp.

2-8°C

Quality Level

200

General description

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Application

Protein A-Sepharose was used:

for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
in immunoprecipitation of [³²P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.

Protein A-Sepharose^™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose^™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Physical form

Supplied as lyophilized powder stabilized with lactose and dextran.

Preparation Note

Swelling: 1 g swells to approx. 4 ml.

Legal Information

Sepharose is a trademark of Cytiva

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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문서 라이브러리 방문

Subcellular distribution and function of Rab3A, B, C, and D isoforms in insulin-secreting cells.

M Iezzi et al.

Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)

Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.

A major myristylated substrate of protein kinase C and protein kinase C itself are differentially regulated during murine B- and T-lymphocyte development and activation.

P Hornbeck et al.

Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)

The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.

Theiler's virus L protein is targeted to the mitochondrial outer membrane.

Frédéric Sorgeloos et al.

Journal of virology, 85(7), 3690-3694 (2011-01-14)

The L protein encoded by Theiler's murine encephalomyelitis virus (TMEV) is a unique example of a picornaviral protein encoded by an alternative open reading frame. This protein is an important determinant of TMEV persistence in the mouse central nervous system.

Proteins from multiple metabolic pathways associate with starch biosynthetic enzymes in high molecular weight complexes: a model for regulation of carbon allocation in maize amyloplasts.

Tracie A Hennen-Bierwagen et al.

Plant physiology, 149(3), 1541-1559 (2009-01-27)

Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes

CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.

V Ossipow et al.

Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8219-8223 (1993-09-01)

The CCAAT/enhancer-binding protein (C/EBP) alpha is a leucine zipper protein that is preferentially expressed in certain cell types, such as adipocytes and hepatocytes. Here we show that C/EBP alpha mRNA is translated into two major proteins, C/EBP-42 and C/EBP-30, that

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문서

Antibody Fragmentation with Pepsin Digestion

Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).

프로토콜

Immunoprecipitation Procedure

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

Protein A-Sepharose™ from Staphylococcus aureus

lyophilized powder

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

form

packaging

storage condition

extent of labeling

technique(s)

color

matrix

matrix activation

matrix attachment

matrix spacer

capacity

solubility

suitability

storage temp.

Quality Level

관련 카테고리

설명

General description

Application

Physical form

Preparation Note

Legal Information

안전성 정보

저장 등급

wgk

flash_point_f

flash_point_c

ppe

문서

시험 성적서(COA)

이 제품을 이미 가지고 계십니까?

관련 참고 논문

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문서

프로토콜

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기술 서비스

Protein A-Sepharose^™ from Staphylococcus aureus