P5282
Phalloidin Peptide
≥90% (HPLC), solid, FITC labeled
동의어(들):
Phalloidin-FITC
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C56H60N10O15S2
Molecular Weight:
1177.26
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
제품 이름
Phalloidin, Fluorescein Isothiocyanate Labeled, sequence Amanita phalloides(synthetic: peptide sequence)
biological source
sequence from Amanita phalloides (synthetic: peptide sequence)
form
solid
fluorescence
λex 495 nm; λem 520 nm(lit.)
storage temp.
−20°C
Quality Level
General description
Phalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide, which interacts with actin and this was first identified in phalloidin-poisoned rats. It is a heptapeptide, cyclic in nature, with a crosslink between tryptophan at position 6 and cysteine at position 3. The side chain of amino acid 7 (γ-δ-dihydroxyleucine) in phalloidin, is accessible to modifications, through which fluorescently labelled phalloidin compounds can be produced.
Application
Phalloidin, Fluorescein Isothiocyanate Labeled has been used:
- To visualize F-Actin reorganization in primary neonatal cardiomyocytes (PNCMs) and H9C2 cells (rat cardiac myoblasts) following endothelin-1 (ET-1) and angiotensin II (Ang II) treatment.
- In immunochemistry to label microfilament.
- In immunofluorescence analysis to stain F-actin.
Biochem/physiol Actions
Phalloidin interacts with polymeric actin, and not oligomeric or monomeric forms. This interaction leads to highly stabilized actin filaments, which resist depolymerization and disassembly. In rats, this toxin causes death due to liver hemorrhage, and cells show abnormal actin clustering. The affinity of phalloidin to actin is not significantly altered after derivatizing fluorescently labelled phalloidin compounds. These compounds can be used to study actin structure and organization within eukaryotic cells.
Toxin that binds polymeric F actin, stabilizing it and interfering with the function of actin-rich structures.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
저장 등급
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Non-muscle myosin heavy chain as a possible target for protein encoded by metastasis-related mts-1 gene.
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The culture of fibroblasts from diaphragm of giant panda.
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Ting Xu et al.
Journal of leukocyte biology, 84(4), 1192-1201 (2008-07-26)
Recruitment of leukocytes onto inflamed tissues is an important physiological event, in which L-selectin plays an essential role in initial leukocyte capture and at the same time, triggers cell signaling. Lck is a member of the Src family of protein
Fluorescent phallotoxin, a tool for the visualization of cellular actin.
Wulf E
Proceedings of the National Academy of Sciences of the USA, 76(9), 4498-4502 (1979)
The H9C2 cell line and primary neonatal cardiomyocyte cells show similar hypertrophic responses in vitro.
Watkins SJ
In Vitro Cellular & Developmental Biology. Animal, 47(2), 125-131 (2011)
문서
In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
관련 콘텐츠
Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.
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