P5380
Protease from Bacillus licheniformis
Type VIII, lyophilized powder, 7-15 units/mg solid
동의어(들):
Proteinase from Bacillus licheniformis, Subtilisin A
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-752-2
MDL number:
Specific activity:
7-15 units/mg solid
Biological source:
Bacillus licheniformis
제품 이름
Protease from Bacillus licheniformis, Type VIII, lyophilized powder, 7-15 units/mg solid
biological source
Bacillus licheniformis
type
Type VIII
form
lyophilized powder
specific activity
7-15 units/mg solid
mol wt
27 kDa
purified by
crystallization
solubility
10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear
H2O: soluble, colorless
foreign activity
DNase ≤5.0 Kunitz units/mg solid
RNase ≤0.05 Kunitz units/mg solid
storage temp.
−20°C
Quality Level
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관련 카테고리
Application
The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies.
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.
Biochem/physiol Actions
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease, Product P5380, is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is active in some organic solvents such as dry octane.
General description
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
The enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60 °C.
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
저장 등급
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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The Journal of physiology, 586(6), 1755-1766 (2008-02-02)
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American journal of respiratory and critical care medicine, 202(12), 1636-1645 (2020-07-30)
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Shannon E Campbell et al.
The Journal of biological chemistry, 279(35), 36235-36241 (2004-05-27)
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프로토콜
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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