P5994
Phosphatase substrate
40 mg tablets
동의어(들):
4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS 번호:
Molecular Weight:
371.14
NACRES:
NA.54
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
form
tablet
concentration
36-44 mg/tablet
impurities
p-nitrophenol, essentially free
solubility
H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
storage temp.
−20°C
Quality Level
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General description
Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.
Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).
The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.
Preparation Note
Contains 40 mg substrate per tablet
Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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