P8169
Phytagel™
BioReagent, suitable for plant cell culture, powder
동의어(들):
Gellan Gum, Agar substitute gelling agent
product line
BioReagent
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
Quality Level
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General description
Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.
Application
Phytagel™ has been used:
- in the solidification of culture medium for root assays in Arabidopsis thaliana
- as a component of Solid Paul′s medium for Agrobacterium culture
- as a component of root induction medium in Ilex dumosa tissue culture
Biochem/physiol Actions
Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.
Preparation Note
To prevent clumping, add slowly to rapidly stirring medium prior to heating.
Other Notes
An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.
Legal Information
Phytagel is a trademark of Sigma-Aldrich Co. LLC
저장 등급
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Cytoskeletal dynamics in interphase, mitosis and cytokinesis analysed through Agrobacterium-mediated transient transformation of tobacco BY-2 cells
Buschmann H, et al.
The New phytologist, 190(1), 258-267 (2011)
Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system
Luna C, et al.
Biocell, 27(2), 205-212 (2003)
Abhishek Bhaskaran et al.
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology, 19(5), 874-880 (2016-05-22)
Longer procedural time is associated with complications in radiofrequency atrial fibrillation ablation. We sought to reduce ablation time and thereby potentially reduce complications. The aim was to compare the dimensions and complications of 40 W/30 s setting to that of
CEP genes regulate root and shoot development in response to environmental cues and are specific to seed plants
Delay C, et al.
Journal of Experimental Botany, 64(17), 5383-5394 (2013)
Rory N Pruitt et al.
The New phytologist, 215(2), 725-736 (2017-05-31)
The biotrophic pathogen Xanthomonas oryzae pv. oryzae (Xoo) produces a sulfated peptide named RaxX, which shares similarity to peptides in the PSY (plant peptide containing sulfated tyrosine) family. We hypothesize that RaxX mimics the growth-stimulating activity of PSY peptides. Root length
프로토콜
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
관련 콘텐츠
Instructions
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