R7876
Ribonucleic acid, transfer from wheat germ
Type V, 15-19 units/mg solid
동의어(들):
Transfer RNA, tRNA
grade
Molecular Biology
type
Type V
form
lyophilized powder
specific activity
15-19 units/mg solid
storage condition
desiccated
solubility
H2O: ~2 mg/mL, clear to hazy
storage temp.
2-8°C
Quality Level
General description
Wheat germ tRNA contains many different specific tRNA molecules.
Application
Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.
Preparation Note
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Other Notes
One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening.
Bernard Thisse et al.
Methods in cell biology, 77, 505-519 (2004-12-18)
Christèle Maison et al.
Nature genetics, 30(3), 329-334 (2002-02-19)
Post-translational modification of histone tails is thought to modulate higher-order chromatin structure. Combinations of modifications including acetylation, phosphorylation and methylation have been proposed to provide marks recognized by specific proteins. This is exemplified, in both mammalian cells and fission yeast
Yana P Blokhina et al.
PLoS genetics, 15(1), e1007730-e1007730 (2019-01-18)
Meiosis is a cellular program that generates haploid gametes for sexual reproduction. While chromosome events that contribute to reducing ploidy (homologous chromosome pairing, synapsis, and recombination) are well conserved, their execution varies across species and even between sexes of the
Ting Shuang Pan et al.
Folia parasitologica, 60(3), 181-191 (2013-08-21)
Acanthocephalans are a small group of obligate endoparasites. They and rotifers are recently placed in a group called Syndermata. However, phylogenetic relationships within classes of acanthocephalans, and between them and rotifers, have not been well resolved, possibly due to the
Biochemistry: The ylide has landed.
Bradley J Landgraf et al.
Nature, 498(7452), 45-47 (2013-05-17)
관련 콘텐츠
Instructions
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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