S8761
Sodium Bicarbonate
Solution (7.5%), sterile-filtered, suitable for cell culture
동의어(들):
aqueous sodium bicarbonate
제품 이름
Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
SMILES string
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
sterility
sterile-filtered
product line
BioReagent
form
solution (7.5%)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.8-8.2
storage temp.
2-8°C
Quality Level
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General description
Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.
Application
Sodium bicarbonate solution has been used:
- as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
- as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
- as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
- to prepare collagen mix droplets for 3D collagen sprouting assay
- to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture
Preparation Note
Prepared in cell culture grade water.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Phenotypic Modulation of Adipose-Derived Stem Cells and Fibroblasts Treated with Povidone--Iodine and Chlorhexidine in Mono and Coculture Models
Chelmu? BA, et al.
Biomedicines, 11(7), 1855-1855 (2023)
Nicoline Dorothea Jakobsen et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 178, 106282-106282 (2022-08-23)
Rat epidermal keratinocyte (REK) Organotypic culture (ROC) is an epidermis model that is robust and inexpensive to develop and maintain, and it has in previous studies been shown to have permeability characteristics close to those of human skin. Here, we
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
Anna E Engler et al.
Cell reports, 33(13), 108553-108553 (2020-12-31)
There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells
Caroline Volz et al.
Cell reports, 31(4), 107568-107568 (2020-04-30)
Anti-angiogenic treatment targeting vascular endothelial growth factor (VEGF)-VEGFR2 signaling has shown limited efficacy in lung cancer patients. Here, we demonstrate that inhibition of VEGFR2 in tumor cells, expressed in ∼20% of non-squamous non-small cell lung cancer (NSCLC) patients, leads to
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