S9754
Sulfatase from abalone entrails
Type VIII, lyophilized powder, 20-40 units/mg solid
동의어(들):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-772-1
MDL number:
Specific activity:
20-40 units/mg solid
Biological source:
abalone entrails
biological source
abalone entrails
type
Type VIII
form
lyophilized powder
specific activity
20-40 units/mg solid
mol wt
~43 kDa by gel filtration
foreign activity
β-Glucuronidase ≤10.0 Sigma units/mg solid
storage temp.
−20°C
Quality Level
Application
Sulfatase from abalone entrails has been used in a study to determine that human p-selectin glycoprotein ligand-1 interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. Sulfatase from abalone entrails has also been used in a study to investigate HPLC of sulfate and glutathione conjugates from hamster embryo fibroblasts.
Sulfatase from abalone entrails has been used to digest the conjugated forms into free forms for the analysis of tissue green tea polyphenols (GTPs). It has also been used for the detection of free, glucuronidated or total form of GTPs.
Biochem/physiol Actions
Sulfatases hydrolyze the sulfate esters of complex macromolecules, like glycosaminoglycans and sulfatides. It helps to modulate the sulfation states that determine the action of several physiological molecules.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Quercetin increased bioavailability and decreased methylation of green tea polyphenols in vitro and in vivo
Wang P, et al.
Food, 3(6), 635-642 (2012)
Sulfatases: structure, mechanism, biological activity, inhibition, and synthetic utility
Hanson SR, et al.
Angewandte Chemie (International ed. in English), 43(43), 5736-5763 (2004)
B A Merrick et al.
Carcinogenesis, 6(9), 1303-1307 (1985-09-01)
Chromatography of benzo[a]pyrene (BaP) sulfate, glucuronide and glutathione (GSH) conjugate standards were examined by h.p.l.c. on a C8 column as modified by various organic acids and solvents. Sulfate and glucuronide standards were positional isomers derived from BaP-1,3,6,7,9 phenols and BaP-GSH
Use of a Phage Display Antibody to Measure the Enzymatic Activity of the Sulfs
Uchimura K, et al.
Test, 480, 51-64 (2010)
Jennifer L Donovan et al.
The British journal of nutrition, 87(1), 31-37 (2002-03-16)
Little is known about flavonoid metabolism and excretion in man. In the present study, the urinary excretion of a major flavonoid in wine, catechin, and its metabolites, were measured after nine human subjects each consumed 120 ml red wine (RW)
프로토콜
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
관련 콘텐츠
Instructions
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