T2694
Trizma® hydrochloride solution
pH 8.0, BioPerformance Certified, 1 M, suitable for cell culture
동의어(들):
Tris hydrochloride solution
grade
BioPerformance Certified, Molecular Biology
form
solution
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)
pH
8.0
useful pH range
7.0-9.0
absorption
≤0.05 at 290 at 40%
Quality Level
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General description
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Application
Trizma® hydrochloride solution has been used:
- as a component of extraction buffer for Plasmodiophora brassicae DNA isolation
- as a component of resuspension buffer for human genomic DNA fragmented sample
- for adjusting pH of plasma samples and as a component of reconstitution and neutralization buffer
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Anti-peptide monoclonal antibodies generated for immuno-MRM assays have a high probability of supporting Western blot and ELISA
Schoenherr RM, et al.
Molecular and Cellular Proteomics, mcp-O114 (2014)
Olivier Harismendy et al.
Genome biology, 12(12), R124-R124 (2011-12-22)
Ultra-deep targeted sequencing (UDT-Seq) can identify subclonal somatic mutations in tumor samples. Early assays' limited breadth and depth restrict their clinical utility. Here, we target 71 kb of mutational hotspots in 42 cancer genes. We present novel methods enhancing both
Accurate variant detection across non-amplified and whole genome amplified DNA using targeted next generation sequencing
ElSharawy A, et al.
BMC Genomics, 13(1), 500-500 (2012)
Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
Mehrabi S, et al.
Bio-protocol (2018)
Markus Frederik Schliffka et al.
eLife, 10 (2021-04-20)
During the first days of mammalian development, the embryo forms the blastocyst, the structure responsible for implanting the mammalian embryo. Consisting of an epithelium enveloping the pluripotent inner cell mass and a fluid-filled lumen, the blastocyst results from a series
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