T3411
Tetramethylammonium chloride solution
Molecular Biology
동의어(들):
N,N,N-Trimethylmethanaminium chloride
InChI
1S/C4H12N.ClH/c1-5(2,3)4;/h1-4H3;1H/q+1;/p-1
SMILES string
[Cl-].C[N+](C)(C)C
InChI key
OKIZCWYLBDKLSU-UHFFFAOYSA-M
grade
Molecular Biology
concentration
5 M
foreign activity
DNase, RNase, none detected
Quality Level
General description
Tetramethylammonium binds AT-rich DNA polymers while concomitantly abolishing the preferential melting of AT versus GC base pairs. It is supplied as a 0.2 μm filtered solution in 18 megohm water.
Application
Tetramethylammonium chloride solution (TMAC) has been used:
- in the preparation of hybridization cocktail for array hybridization and scanning
- in next-generation sequencing (NGS), and genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) library preparation
- in the preparation of TMAC buffer and bead hybridization mixture for hybridization and detection
Nikolay L Malinin et al.
Nature protocols, 16(12), 5592-5615 (2021-11-14)
Genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) is a sensitive, unbiased, genome-wide method for defining the activity of genome-editing nucleases in living cells. GUIDE-seq is based on the principle of efficient integration of an end-protected double-stranded oligodeoxynucleotide
W B Melchior et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(2), 298-302 (1973-02-01)
Several small alkylammonium ions can eliminate, or even reverse, the usual dependence of the DNA transition temperature on base composition. For example, in 3 M tetramethylammonium chloride, or 2.4 M tetraethylammonium chloride, DNAs of different base compositions all melt at
Yanwen Guo et al.
Methods in molecular biology (Clifton, N.J.), 1176, 33-44 (2014-07-18)
As small noncoding RNAs, microRNAs (miRNAs) regulate diverse biological functions, including physiological and pathological processes. The expression and deregulation of miRNA levels contain rich information with diagnostic and prognostic relevance and can reflect pharmacological responses. The increasing interest in miRNA-related
Hybridization of genomic DNA to oligonucleotide probes in the presence of tetramethylammonium chloride.
A G DiLella et al.
Methods in enzymology, 152, 447-451 (1987-01-01)
Xiao-Yong Li et al.
Methods in molecular biology (Clifton, N.J.), 809, 3-26 (2011-11-25)
Immunoprecipitation of cross-linked chromatin in combination with microarrays (ChIP-chip) or ultra high-throughput sequencing (ChIP-seq) is widely used to map genome-wide in vivo transcription factor binding. Both methods employ initial steps of in vivo cross-linking, chromatin isolation, DNA fragmentation, and immunoprecipitation.
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