제품 이름
Anti-β-Tubulin Isotype III antibody, Mouse monoclonal, clone SDL.3D10, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SDL.3D10, monoclonal
form
buffered aqueous solution
species reactivity
mouse, rat, human, boar, bovine
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using rat brain extract
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)
Application
Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse has been used in immunolabelling and flow cytometry.
Monoclonal anti-β-tubulin isotype III antibody (diluted 1:500) can be used as a primary antibody in immunocytochemical analysis of neurons. It can also be used for the localization of isotype III of β-tubulin by several immunochemical assays like dot blot, ELISA and immunohistochemistry.
Biochem/physiol Actions
Tubulin β 3 class III (TUBB3) regulates neurogenesis and is involved in axon guidance and maintenance. Mutations in this gene is associated with hypoplasia of commissural axon (CA), congenital fibrosis of the extraocular muscles type 3 (CFEOM3) and dysgyria.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Tubulin β 3 class III (TUBB3) encodes for the microtubule protein tubulin β‐3, which is majorly expressed in neurons. The gene is located on human chromosome 16q24.3.
Tubulin is the main constituent of microtubules made up of two similar polypeptides α and β, each with 50 kD (approx.) molecular weight. It has several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin isotype III antibody can be used in microarray and western blotting. It can also be used in immunoblotting. Mouse anti-β-tubulin isotype III antibody reacts specifically with an epitope present on the β-tubulin (isotype III) of bovine, rat, mouse, boar and human but not with tubulin present in chicken brain preparation or in a chicken fibroblasts cell line.
Immunogen
peptide corresponding to the carboxy-terminal sequence of human β-tubulin isotype III, conjugated to BSA.
Physical form
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
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저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Evaluation of tubulin beta-3 as a novel senescence-associated gene in melanocytic malignant transformation
Orfanidis K, et al.
Pigment Cell & Melanoma Research, 30(2), 243-254 (2017)
Cytoskeleton in action: lissencephaly, a neuronal migration disorder
Moon HM and Wynshaw-Boris A
Wiley interdisciplinary reviews. Developmental biology, 2(2), 229-245 (2013)
Human TUBB3 Mutations Disrupt Netrin Attractive Signaling
Huang H, et al.
Neuroscience, 374, 155-171 (2018)
Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin.
A Banerjee et al.
The Journal of biological chemistry, 265(3), 1794-1799 (1990-01-25)
Tubulin, the major constituent protein of microtubules, is a heterodimer of alpha and beta subunits. Both alpha and beta exist in multiple isotypic forms. It is not clear if different isotypes perform different functions. In order to approach this question
J Pĕknicová et al.
Biology of reproduction, 65(3), 672-679 (2001-08-22)
The exposure of tubulin epitopes was studied in ejaculated boar spermatozoa using a panel of four monoclonal antibodies specific to the N-terminal or C-terminal structural domains of tubulin and three monoclonal antibodies against class III beta-tubulin. The specificity of the
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