T5293
Monoclonal Anti-β-Tubulin antibody produced in mouse
clone 2-28-33, ascites fluid
동의어(들):
Anti-β-Tubulin Antibody
제품 이름
Monoclonal Anti-β-Tubulin antibody produced in mouse, clone 2-28-33, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
2-28-33, monoclonal
contains
15 mM sodium azide
species reactivity
Ciona intestinalis, rat, chicken, Caenorhabditis elegans, Strongylocentrotus purpuratus, bovine, human
enhanced validation
independent ( Antibodies)
Learn more about Antibody Enhanced Validation
technique(s)
indirect immunofluorescence: 1:1,000 using cultured chicken fibroblasts or cultured human foreskin fibroblasts
western blot: 1:100
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
rat ... Tubb2(29212), Tubb2c(296554)
Application
Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting and fluorescence immunocytochemical staining.
Biochem/physiol Actions
Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins.Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).
Immunogen
sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
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저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Birth of Piglets After Transfer of Embryos Cryopreserved by Cytoskeletal Stabilization and Vitrification
Dobrinsky JR
Biology of Reproduction, 62, 564-570 (2000)
Hanna S Radomska et al.
The Journal of clinical investigation, 122(8), 2955-2966 (2012-07-17)
Mutations that activate the fms-like tyrosine kinase 3 (FLT3) receptor are among the most prevalent mutations in acute myeloid leukemias. The oncogenic role of FLT3 mutants has been attributed to the abnormal activation of several downstream signaling pathways, such as
Tilman Jobst-Schwan et al.
American journal of physiology. Renal physiology, 305(5), F734-F744 (2013-07-05)
The inhibitor of apoptosis protein survivin is a bifunctional molecule that regulates cellular division and survival. We have previously shown that survivin protein can be found at high concentrations in the adult kidney, particularly in the proximal tubules. Here, survivin
Thomas Stahnke et al.
PloS one, 12(2), e0172592-e0172592 (2017-02-24)
In glaucoma surgery, fibrotic processes occur, leading to impairment of liquid outflow. Activated fibroblasts are responsible for postoperative scarring. The transforming growth factor-β (TGF-β) pathway plays a key role in fibroblast function, differentiation and proliferation. The aim of this study
Beta-tubulin binds Src homology 2 domains through a region different from the tyrosine-phosphorylated protein-recognizing site.
Itoh T
The Journal of Biological Chemistry, 271, 27931-27935 (1996)
문서
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
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