T5525
3,3′,5,5′-Tetramethylbenzidine
TMB substrate, chromogenic, tablet
동의어(들):
BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS 번호:
Molecular Weight:
240.34
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
2808541
제품 이름
3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
SMILES string
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
form
tablet
mp
168-171 °C (lit.)
storage temp.
2-8°C
Quality Level
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Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Lactobacillus casei LcY decreases milk protein immunoreactivity of fermented buttermilk but also contains IgE-reactive proteins
Wroblewska B, et al.
Food Research International, 83, 95-101 (2016)
Effect of acylation and enzymatic modification on pea proteins allergenicity
Szymkiewicz, A and Jedrychowski, L
Polish Journal of Food and Nutrition Sciences, 58(3) (2008)
Immunoreactive properties of peptide fractions of cow whey milk proteins after enzymatic hydrolysis
Wroblewska B, et al.
Polish Journal of Food and Nutrition Sciences, 39(8), 839-850 (2004)
Ronit Mazor et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(23), 8571-8576 (2014-05-07)
Nonhuman proteins have valuable therapeutic properties, but their efficacy is limited by neutralizing antibodies. Recombinant immunotoxins (RITs) are potent anticancer agents that have produced many complete remissions in leukemia, but immunogenicity limits the number of doses that can be given
Simon Z He et al.
Leukemia & lymphoma, 56(5), 1406-1415 (2014-09-25)
Acute myeloid leukemia (AML) blasts express high levels of interlekin-3 (IL-3) receptor-α (CD123). CSL360 is a recombinant, chimeric immunoglobulin G1 (IgG1), anti-CD123 monoclonal antibody (MoAb) that neutralizes IL-3 and demonstrates anti-leukemic activity in vitro. This phase 1 study assessed safety
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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