Anti-TGN46 antibody produced in rabbit

Anti-TGN46 recognizes human trans-golgi network protein 2 (TGN46). Detection of the TGN46 band by immunoblotting is specifically inhibited by the immunizing peptide.

TGN46 (Trans-Golgi network protein, 46 kDa), the human homologue of rat TGN38, is a resident integral membrane protein of the trans-Golgi network (TGN) that cycles constitutively between the TGN and the plasma membrane, returning via endosomes. The TGN is the major sorting compartment of the secretory pathway for proteins, lipids and membrane traffic. TGN46 is a heavily glycosylated protein, probably involved in regulating membrane traffic to and from the TGN. TGN46 contains a signal peptide, lumenal domain, membrane-spanning domain, and cytoplasmic domain. The membrane spanning region and cytoplasmic tail contain the retention and retrieval signals, respectively for localization in the TGN. TGN46 is widely expressed.

synthetic peptide corresponding to amino acid residues 426-437 of human TGN46 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in monkey and differs by three amino acids in rat and mouse.

Rabbit polyclonal anti-TGN46 antibody is used to tag trans-golgi network protein 2 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of trans-golgi network protein 2 as a membrane traffic regulating component of the trans-Golgi network (TGN). Anti-TGN46 may be used as a TGN marker.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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