T8203
Anti-TUBA1A (TUBA3) Antibody
mouse monoclonal, AA13
동의어(들):
Anti-Tuba1a, Anti-Tubulin, α1A
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
AA13, monoclonal
Application:
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
indirect ELISA
western blot
immunohistochemistry
immunoprecipitation (IP)
indirect ELISA
western blot
Species reactivity:
human, rat, mouse
Citations:
183
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract
Uniprot accession no.:
제품 이름
Anti-α-Tubulin antibody, Mouse monoclonal, clone AA13, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AA13, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
human, rat, mouse
packaging
antibody small pack of 25 μL
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~2.0 mg/mL
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBA1A(7846)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
Application
Anti-α-Tubulin antibody, Mouse monoclonal has been used in western blotting.
The antibody has been used in various immunochemical techniques including immunoprecipitation, immunocytochemistry, and immunohistochemistry.
Biochem/physiol Actions
Gene mutation of tubulin α-1A (TUBA1A) is observed in perisylvian asymmetrical polymicrogyria, polymicrogyria-like cortical dysplasia, and microlissencephaly in foetal cases. Alteration in the expression of the gene impairs neural migration and causes lissencephaly (LIS).
Tubulin is the major building block of microtubules, which is a structural and mobile element in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Monoclonal antibodies recognizing α-tubulin, together with monoclonal antibodies to other tubulin types (α-tubulin isotypes I, II, and III, tyrosine tubulin and acetylated- α -tubulin) provide a specific and useful tool in studying the intracellular distribution of tubulin, and the static and dynamic aspects of the cytoskeleton. In addition, the various tubulin forms have been implicated in cancerous processes and are thus suggested as potential targets for cancer therapy.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma AA13 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with rat brain tubulin.
Tubulin α 1a (TUBA1A) is a member of α-tubulin gene family. It is encoded by the gene mapped to human chromosome 12q13.12. Tubulin α 1a is a vital structural subunit of microtubules that are temporarily expressed in almost all post-mitotic neurons during neuronal development.
The encoded protein structure contains core of two β-sheets enclosed by α-helices. TUBA1A is characterized with an amino-terminal domain with the guanine nucleotide-binding region, an intermediate domain associated with interprotofilament lateral contacts and the modulation of tubulin curvature, and a carboxy-terminal domain that contains region involved in interacting with microtubule-associated proteins (MAPs) and molecular motors, such as kinesins and dynein.
The encoded protein structure contains core of two β-sheets enclosed by α-helices. TUBA1A is characterized with an amino-terminal domain with the guanine nucleotide-binding region, an intermediate domain associated with interprotofilament lateral contacts and the modulation of tubulin curvature, and a carboxy-terminal domain that contains region involved in interacting with microtubule-associated proteins (MAPs) and molecular motors, such as kinesins and dynein.
Tubulin is a cylindrical, filamentous structure is present inalmost all eukaryotic cells.
Immunogen
rat brain tubulin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
Ahlskog JK
Molecular and Cellular Biology, 30(24), 5608-5620 (2010)
New tubulin targeting agents currently in clinical development
Carlson RO
Expert Opinion on Investigational Drugs, 17(5), 707-722 (2008)
TUBA1A mutation can cause a hydranencephaly-like severe form of cortical dysgenesis.
Yokoi S
Scientific Reports, 5:15165 (2015)
A case of TUBA1A mutation presenting with lissencephaly and Hirschsprung disease.
Brain & Development, 36(2), 159-162 (2014)
Expanding the spectrum of TUBA1A-related cortical dysgenesis to Polymicrogyria.
Poirier K
European Journal of Human Genetics, 21(4), 381-385 (2013)
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