V9131

Anti-Vinculin Antibody

Name: Anti-Vinculin Antibody
Brand: SIGMA

mouse monoclonal, hVIN-1

동의어(들):

Anti Vinculin, Vinculin Antibody, Vinculin Antibody - Monoclonal Anti-Vinculin antibody produced in mouse

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162905

주요 문서

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제품 이름

Monoclonal Anti-Vinculin antibody produced in mouse, clone hVIN-1, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

hVIN-1, monoclonal

mol wt

antigen 116 kDa

contains

15 mM sodium azide

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:400 using cultured human fibroblasts
western blot: 1:200 using extract of human fibroblasts

isotype

IgG1

UniProt accession no.

P18206

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

Application

Monoclonal Anti-Vinculin antibody produced in mouse has been used in:

western blotting
immunofluorescence staining
immunohistochemistry

Biochem/physiol Actions

Monoclonal Anti-Vinculin specifically stains vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells using indirect immunofluorescent labeling. The antibody reacts with the 116 kDa vinculin band in immunoblotting. The product reacts with vinculin of many species. Good reactivity is obtained with human, bovine, chicken, dog, rat, mouse, turkey, and Xenopus. The antibody shows cross reactivity with smooth muscle metavinculin.

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the β-subunit of integrin binds to talin. Talin binds to vinculin that interacts with α-actinin and possibly with itself. Since α-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions and the membrane-associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Immunogen

purified human vinculin from uterus.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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저장 등급

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Vinculin and talin: focus on the myocardium

Zemljic-Harpf A, et al.

Journal of Investigative Medicine, 57(8), 849-855 (2009)

Prognostic significance of epidermal growth factor receptor phosphorylation and mutation in head and neck squamous cell carcinoma

Hama T, et al.

Oncologist, 14(9), 900-908 (2009)

Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure

Minamishima YA, et al.

Blood, 111(6), 3236-3244 (2008)

Reinforcement versus fluidization in cytoskeletal mechanoresponsiveness

Krishnan R, et al.

PLoS ONE, 4(5), e5486-e5486 (2009)

Dystrophin-glycoprotein complex and vinculin-talin-integrin system in human adult cardiac muscle

Anastasi G, et al.

International Journal of Molecular Medicine, 23(2), 149-159 (2009)

모든 관련된 서류 보기

문서

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Antibody Generation | Monoclonal vs Polyclonal Antibodies

Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.

Tips for Immunofluorescence Protocols

Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.

Antibody Basics

구조, 클래스, 면역글로불린의 정상 범위에 관한 기술 정보 등 항체 작업의 기본 원칙에 대해 알아보십시오.

모두 보기

프로토콜

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

Anti-Vinculin Antibody

mouse monoclonal, hVIN-1

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

biological source

conjugate

antibody form

antibody product type

clone

mol wt

contains

species reactivity

enhanced validation

technique(s)

isotype

UniProt accession no.

application(s)

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

관련 카테고리

설명

Application

Biochem/physiol Actions

Disclaimer

General description

Immunogen

Other Notes

안전성 정보

저장 등급

wgk

flash_point_f

flash_point_c

문서

시험 성적서(COA)

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