V9264
Anti-Vinculin Antibody
mouse monoclonal, hVIN-1
동의어(들):
Monoclonal Anti-Vinculin antibody produced in mouse
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
hVIN-1, monoclonal
Application:
immunocytochemistry
immunohistochemistry
indirect ELISA
western blot
immunohistochemistry
indirect ELISA
western blot
Species reactivity:
frog, chicken, mouse, canine, human, bovine, rat, turkey
Citations:
306
Technique(s):
immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.05-0.1 μg/mL using HS-68 total cell extract
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.05-0.1 μg/mL using HS-68 total cell extract
Uniprot accession no.:
제품 이름
Anti-Vinculin antibody, Mouse monoclonal, clone hVIN-1, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
hVIN-1, monoclonal
form
buffered aqueous solution
mol wt
antigen 116 kDa
species reactivity
frog, chicken, mouse, canine, human, bovine, rat, turkey
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.05-0.1 μg/mL using HS-68 total cell extract
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
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Application
Anti-Vinculin antibody, Mouse monoclonal has been used in
- immunoblotting
- immunofluorescence staining
- immunocytochemistry
- immunohistochemistry and enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.
Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.
Immunogen
purified human vinculin from uterus.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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저장 등급
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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