W1878
Williams’ Medium E
With sodium bicarbonate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
동의어(들):
Williams’ E medium
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제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352207
NACRES:
NA.75
제품 이름
Williams′ Medium E, With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
General description
Williams′ Medium E was developed by Williams and Gunn as a modification to Williams′ Medium D. The medium is formulated to support long-term cell culture of adult liver epithelial cells. It is a modified Minimum Essential Medium (MEM) with altered glucose and amino acid content.
Application
Williams′ Medium E has been used in the culturing of human hepatocellular carcinoma (HCC) cells and hepatic cells.
Preparation Note
Supplement with 0.292 g/L L-glutamine.
Other Notes
Phenol red has been shown to interfere with the growth of some cells at low or cloning densities. Use this version of William's E when working with stem cells or cells at low densities.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
관련 콘텐츠
Instructions
Chromatin recruitment of activated AMPK drives fasting response genes co-controlled by GR and PPARα.
Dariusz Ratman et al.
Nucleic acids research, 44(22), 10539-10553 (2016-09-01)
Adaptation to fasting involves both Glucocorticoid Receptor (GRα) and Peroxisome Proliferator-Activated Receptor α (PPARα) activation. Given both receptors can physically interact we investigated the possibility of a genome-wide cross-talk between activated GR and PPARα, using ChIP- and RNA-seq in primary
Robert D Mitchell et al.
Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on
Nicole A Kratochwil et al.
The AAPS journal, 19(2), 534-550 (2017-01-05)
Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2