L7025
LB Broth with agar (Lennox)
Tablet microbial growth medium
Sinónimos:
LB Agar, LB agar
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NACRES:
NA.85
UNSPSC Code:
12352200
Servicio técnico
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Molecular Biology
Quality Level
sterility
non-sterile
form
tablet
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Features and Benefits
Lennox LB tablet with agar provides:
- A budget-friendly alternative to pre-poured plates
- Convenient small package to eliminate weighing
- Fast preparation – no stirring required
Preparation Note
Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.
Other Notes
13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents
Legal Information
Zeocin is a registered trademark of Cayla Sarl
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
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