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Merck

L7275

LB Broth (Lennox)

Tablet microbial growth medium

Sinónimos:

Lennox broth , Luria Bertani Broth

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NACRES:
NA.85
UNSPSC Code:
12352200
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grade

Molecular Biology

Quality Level

sterility

non-sterile

form

tablet

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

LB Broth (Lennox) has been used for culturing Escherichia coli and bacterial artificial chromosomes (BACs).
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox LB tablets provide:
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

Preparation Note

Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Other Notes

10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents

Legal Information

Zeocin is a registered trademark of Cayla Sarl


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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