A8919
Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sinónimos:
Anti Goat Hrp Sigma
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Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p), WB CL
Citations:
65
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Permítanos ayudarlebiological source
rabbit
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:30,000, dot blot: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot (chemiluminescent): 1:160,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. Immunoglobulin g (IgG) is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. Rabbit anti-Goat IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The conjugate is provided as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT as a preservative.
Immunogen
Purified goat IgG
Application
Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reaction.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
12 - Non Combustible Liquids
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