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Merck

D2429

DMEM - low glucose

With sodium pyruvate, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable fore cell culture

Sinónimos:

DME, DMEM

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UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217342

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Nombre del producto

Medio de Eagle modificado de Dulbecco, poca glucosa, 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Application

Dulbecco′s Modified Eagle′s Medium-low glucose has been used:
  • during organoid embedding for neutralizing thawed collagen I
  • to culture melanoma cancer cell lines
  • to store the cancer tissue samples
  • as a differentiation culture medium for C2C12 cells

General description

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of basal medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose.

Preparation Note

Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBL7902
RNBK8505
RNBJ9318
RNBJ4442
RNBJ2213

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