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Merck

F1141

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

Sinónimos:

Fibronectina

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About This Item

UNSPSC Code:
12352202
eCl@ss:
42020141
NACRES:
NA.75
EC Number:
289-149-2
MDL number:

Nombre del producto

Fibronectina plasma bovino, solution, sterile-filtered, BioReagent, suitable for cell culture

biological source

bovine plasma

sterility

sterile-filtered

product line

BioReagent

form

solution

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

UniProt accession no.

binding specificity

Peptide Source: Collagen

Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

2-8°C

Quality Level

Gene Information

bovine ... FN1(280794)

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Analysis Note

La homogeneidad proteica se evalúa mediante inmunoelectroforesis.

Application

La fibronectina (FN) plasmática bovina se ha utilizado en:
  • análisis de fibronectina y de adsorción (ELISA (enzimoinmunoanálisis de absorción))
  • migración transpocillos
  • ensayo de fibronectina

Biochem/physiol Actions

Las fibronectinas participan en la adhesión celular, la organización del citoesqueleto, la transformación oncógena, la migración celular, la fagocitosis, la hemostasia y la diferenciación embrionaria.

General description

La fibronectina es una proteína ubicua de la matriz extracelular. Es una proteína mosaico compuesta por tres tipos de secuencias de aminoácidos consenso modulares que se repiten. Las fibronectinas pertenecen a una clase de glucoproteínas de elevado peso molecular. La fibronectina celular es expresada por los fibroblastos y la fibronectina plasmática suele ser liberada por los hepatocitos.

Other Notes

El producto F1141, la fibronectina del plasma bovino es una disolución en TBS 0,05 M a pH 7,5.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Certificados de análisis (COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I choose which fibronectin product to use to coat culture surfaces for cell growth?

    The species source for the fibronectin used when coating culture surfaces is not important.  It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.

  4. How long can I store the fibronectin solution?

    The product is good for at least a year at 2-8 °C if kept sterile.

  5. How long can I store fibronectin coated plates, etc?

    Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.

  6. What is the procedure for coating culture surfaces with fibronectin?

    To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume.  2. Allow to air dry for at least 45 minutes at room temperature.  Excess fibronectin may be removed by aspiration, but this is not necessary.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 and p42/44MAPK dependent mechanisms
Fang W B, et al.
The Journal of biological chemistry, jbc-M112 (2012)
Enhanced fibronectin adsorption on carbon nanotube/poly (carbonate) urethane: independent role of surface nano-roughness and associated surface energy
Khang D, et al.
Biomaterials, 28(32), 4756-4768 (2007)
FIBRONECTIN AUTOANTIBODIES
Autoantibodies, 221-251 (2007)
Tests to predict preeclampsia
Chesley's Hypertensive Disorders in Pregnancy, 221-251 (2015)
Role of subnano-, nano-and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells
Khang D, et al.
Biomaterials, 33(26), 5997-6007 (2012)

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