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Merck

FLAA

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit

for ATP quantitation

Sinónimos:

ATP Bioluminescence Assay, ATP Determination Kit, Luminescent ATP Detection kit

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EC Number:
213-579-1
NACRES:
NA.26
eCl@ss:
32160414
UNSPSC Code:
41116133

Documentos clave

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storage temp.

−20°C

Quality Level

200

Categorías relacionadas

General description

The Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of ATP in samples. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin. When ATP is the limiting reagent, the light emitted is proportional to the ATP present in the sample.

Application

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit has also been used in the quantification of ATP in 3D matrixes of human neurons, hepatic cells with ischemia, various bacterial cultures and lysosomes.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • FLAABAdenosine 5′-triphosphate (ATP) assay mix dilution buffer, lyophilized powder mL/vialSDS
  • FLAAMAdenosine 5′-triphosphate (ATP) assay mix, lyophilized powder mL/vialSDS
  • FLAASAdenosine 5′-triphosphate (ATP) disodium salt hydrate, vial of ~1 mg ATP mL/vialSDS

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

wgk

WGK 3

Clase de almacenamiento

11 - Combustible Solids

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Certificados de análisis (COA)

Lot/Batch Number
0000502396
0000502395
0000502396
0000502395
0000502399

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  1. How many reactions can one do with Product FLAA, ATP Bioluminescent Assay Kit (one vial of FLAAM)?

    When one reconstitutes the vial to 5 ml, as directed, one can perform 50 assays using 100 ul per test. If one dilutes 1:5 one can get 250 tests per kit.

  2. What is the sensitivity of Product FLAA, ATP Bioluminescent Assay Kit the assay mix?

    With FLAAM diluted 1:5, one can easily measure 1 pmol ATP in 100 μl (10 nM) with 50 μl of the reagent.

  3. What  is the stability of the Product FLAA, ATP Bioluminescent Assay Kit ATP stock solution?

    The ATP standard stock solution (FLAAS) is stable for at least 24 hours at 0-5°C, or for over two weeks if stored frozen at -20°C.

  4. Can Product FLAA, ATP Bioluminescent Assay Kit assay mix be used on cells and tissues?

    This reagent measures only soluble ATP. Cells must be washed and then lysed to obtain a soluble ATP extract. Tissues must be homogenized and clarified to provide an extract. The supernatant solution can then be used for assay.

  5. How can one check for inhibitors in the samples when using Product FLAA, ATP Bioluminescent Assay Kit?

    One must add an amount of ATP from a standard solution equivalent to the ATP already found in the sample. If the signal is doubled, the absence of an inhibitor is indicated.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

F Chen et al.
Journal of clinical microbiology, 32(11), 2791-2800 (1994-11-01)
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Yuri L Boteon et al.
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