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Merck

I3146

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Sinónimos:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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NACRES:
NA.75
UNSPSC Code:
12352205
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Nombre del producto

ITS Liquid Media Supplement (100×), liquid, sterile-filtered, BioReagent, suitable for cell culture

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Quality Level

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells
  • rat fetal testis explants
  • human dental pulp stem cells (DPSC)

Biochem/physiol Actions

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.

Other Notes

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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