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About This Item
Nombre del producto
Protein A–Sepharose™ 6MB, aqueous ethanol suspension
form
aqueous ethanol suspension
extent of labeling
~1 mg per mL
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
~6 mg/mL binding capacity (human IgG)
storage temp.
2-8°C
Quality Level
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Application
Protein A-Sepharose™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Protein A–Sepharose™ 6MB has been used in immunoprecipitation.
General description
P6649-10ml′s updated product number is GE17-0469-01
Physical form
Suspension in 20% ethanol
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Clase de almacenamiento
3 - Flammable liquids
wgk
WGK 3
flash_point_f
100.4 °F
flash_point_c
38 °C
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G V Hillyer et al.
The American journal of tropical medicine and hygiene, 38(3), 547-552 (1988-05-01)
Total RNA containing messenger RNA has been isolated from adult Fasciola hepatica and translated in vitro using the rabbit reticulocyte lysate system. L-[35S]-methionine labeled translation products have been immunoprecipitated with sera collected from rabbits infected with F. hepatica, rabbits immunized
Gaurav Dugar et al.
Molecular cell, 69(5), 893-905 (2018-03-03)
Cas9 nucleases naturally utilize CRISPR RNAs (crRNAs) to silence foreign double-stranded DNA. While recent work has shown that some Cas9 nucleases can also target RNA, RNA recognition has required nuclease modifications or accessory factors. Here, we show that the Campylobacter
Nikolai Peschek et al.
The EMBO journal, 38(16), e101650-e101650 (2019-07-18)
Small regulatory RNAs (sRNAs) are crucial components of many stress response systems. The envelope stress response (ESR) of Gram-negative bacteria is a paradigm for sRNA-mediated stress management and involves, among other factors, the alternative sigma factor E (σE ) and
Verena Pfeiffer et al.
Molecular microbiology, 66(5), 1174-1191 (2007-11-01)
The Salmonella pathogenicity island (SPI-1) encodes approximately 35 proteins involved in assembly of a type III secretion system (T3SS) which endows Salmonella with the ability to invade eukaryotic cells. We have discovered a novel SPI-1 gene, invR, which expresses an
A J Williams et al.
Diabetes care, 24(3), 504-509 (2001-04-06)
To determine the extent of celiac autoimmunity in type 1 diabetic patients and the overlap between islet and celiac autoimmunity in their nondiabetic relatives. IgA antibodies to tissue transglutaminase were determined in serum taken from 433 type 1 diabetic patients
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