S7025
50 bp DNA Step Ladder
for DNA electrophoresis
Sinónimos:
DNA Ladder, Step Ladder, 50 bp, 17 Fragments, 50-3000 bp
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NACRES:
NA.25
UNSPSC Code:
41105335
Servicio técnico
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form
liquid
usage
100 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.
Application
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
Other Notes
Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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