产品名称
抗NG2硫酸软骨素蛋白聚糖抗体, Chemicon®, from rabbit
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, monkey, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CSPG4(1464)
mouse ... Cspg4(121021)
rat ... Cspg4(81651)
rhesus monkey ... Cspg4(713086)
General description
Immunogen
Application
一个先前批次产品已以1:150-1:600的稀释度被用于IC中。
免疫组织化学:
与Alexa Fluor共轭二抗一起,一个先前批次产品以1:200的稀释度已用于胚胎小鼠大脑的冷冻切片中。应注意避免过度固定组织切片。建议在2-4%PFA中进行短暂(5-10分钟)固定。
免疫沉淀:
一个浓度为2 μg/mL的先前批次产品已被用于IP中。
ELISA:
一个先前批次的产品已以1:1,500-1:3,000的稀释度用于ELISA中。
最佳工作稀释度和方案必须由最终用户进行确定
神经 & 胶质标记
神经科学
Biochem/physiol Actions
Physical form
Preparation Note
使用建议:
收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。避免反复冻融循环,以免损坏 IgG 和影响产品性能。
Analysis Note
人黑素瘤、神经胶质瘤和增殖性脑内皮细胞、大鼠B49胶质细胞提取物或胚胎小鼠脑组织。
蛋白质印迹:该批次以1:1000的比例稀释在10 μg大鼠脑裂解液中检测到NG2硫酸软骨素。
Other Notes
Legal Information
Disclaimer
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| AB5320 | 04053252407413 |