产品名称
磷酸盐缓冲溶液, 10× PBS for Western blots and IP
grade
Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
liquid
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
pH
7.2-7.6(1x solution)
storage temp.
room temp
General description
磷酸盐缓冲盐水（PBS）通常用作蛋白质印迹和免疫沉淀（IP）程序中的洗涤缓冲液，以及用于一般分子生物学应用的细胞裂解物的样品制备。
Application
适用于蛋白质印迹和IP的洗涤缓冲液，以及细胞裂解液样品制备。
Preparation Note
10x PBS 含有：
- 30mM 磷酸钠
- 1.5M 氯化钠
- 10.5mM 磷酸钾
使用分子生物级水将 10x PBS 稀释至 1x工作溶液。
Still not finding the right product?
Explore all of our products under 磷酸盐缓冲溶液
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.
Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
Sahar M Greish et al.
International journal of physiology, pathophysiology and pharmacology, 12(5), 134-146 (2020-11-24)
Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney