Quality Level
usage
sufficient for 250 reactions
feature
dNTPs included, hotstart
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified RNA
compatibility
ABI 5700, ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ5, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 s, Eppendorf® Mastercycler ep realplex, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000
detection method
probe-based
shipped in
wet ice
storage temp.
−20°C
General description
Application
Features and Benefits
- 该主混合物（master mix）可确保不同反应之间的一致性和可再现性。
- 降低了多个移液步骤的污染风险
- 与手动或热启动（Hot Start）方法相比，减少了设置时间
- JumpStart™Taq聚合酶可减少引物二聚体和非特异性产物的形成
- 广泛的仪器兼容性
- 包括一个单独的ROX参考染料瓶，用于反应标准化
Packaging
Legal Information
仅试剂盒组分
- Probe Based qRT-PCR ReadyMix 2 X
- Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
signalword
Danger
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
实验方案
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
商品
Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.
分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。
Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.
相关内容
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.