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Merck

A7058

抗多组氨酸−小鼠单克隆甲状腺过氧化物酶抗体 小鼠抗

clone HIS-1, purified from hybridoma cell culture

别名:

聚组氨酸单克隆抗体, 6 His表位标签单克隆抗体, 6xHis标签单克隆抗体, HHHHHH表位标签单克隆抗体, Hexa His标签单克隆抗体, His6标签单克隆抗体, 组氨酸标签单克隆抗体, 聚组氨酸标签单克隆抗体

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关于此项目

NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
HIS-1, monoclonal
Application:
WB
Citations:
152
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biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

storage temp.

2-8°C

target post-translational modification

unmodified

General description

抗多组氨酸过氧化物酶单克隆缀合抗体可识别天然和变性形式的合成多组氨酸或多组氨酸标记的融合蛋白。 该产品可与原核生物pET、pRSET和pTrc表达载体表达的融合蛋白反应。

Immunogen

重组聚组氨酸标签融合蛋白。

Application

抗体适用于免疫印迹。 工作稀释度1:20



也适用于斑点印迹分析和ELISA

Biochem/physiol Actions

该抗体可识别合成聚组氨酸,以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。

Physical form

从含有 1% 牛血清白蛋白和 0.05% MIT的 0.01 M 磷酸盐缓冲盐水 (pH 7.4) 冻干。

Preparation Note

抗体缀合物应用0.5 ml去离子水复溶。
通过Avrameas, S.等人在Scand. J. Immunol., 8, Suppl. 7, 7 (1978)中描述的两步戊二醛法制备。

Legal Information

该产品受专利DE 19507166和国外等效文件的保护,独家授权给德国QIAGEN GmbH、Qiagen Strasse 1、D-40724 Hilden。


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Renhua Huang et al.
PloS one, 11(1), e0145872-e0145872 (2016-01-06)
Affinity reagents of high affinity and specificity are very useful for studying the subcellular locations and quantities of individual proteins. To generate high-quality affinity reagents for human Lyn tyrosine kinase, a phage display library of fibronectin type III (FN3) monobodies
Yang Zhang et al.
Nature communications, 6, 8635-8635 (2015-10-27)
Phenylpropanoids comprise an important class of plant secondary metabolites. A number of transcription factors have been used to upregulate-specific branches of phenylpropanoid metabolism, but by far the most effective has been the fruit-specific expression of AtMYB12 in tomato, which resulted
Vincenzo Lionetti et al.
BMC plant biology, 15, 6-6 (2015-01-20)
Fusarium graminearum, one of the causal agents of Fusarium Head Blight (FHB, scab), leads to severe losses in grain yield and quality due to the production of mycotoxins which are harmful to human and livestock. Different traits for FHB resistance