General description
Sigma的1kb分子量标准含有11个片段,包含0.5至3 kb的500bp重复序列,3至6 kb的1 kb重复序列,以及6至10 kb的2 kb重复序列。在核酸凝胶电泳中,应在凝胶缓冲液中稀释5μl分子量标准,然后在琼脂糖或聚丙烯酰胺凝胶上单泳道上样。适用于Northern和Southern印迹杂交。所有的DNA标记物都可以用溴化乙锭、SYBR™ Green和Nancy-520染色。
Application
1 kb DNA分子量标准品已用作通过凝胶电泳测定双链DNA的分子量和大小的分子量标准。
Other Notes
Sigma的1kb分子量标准品以溶于10mM Tris-HCl(pH 8.0)和1.0 mM EDTA的溶液形式提供。
为实现最佳分辨率,建议采用的琼脂糖凝胶浓度为0.75%。
Legal Information
SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
商品
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
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Instructions
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