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Merck

D4522

脱氧核糖核酸 来源于小牛胸腺

Type XV, Activated, lyophilized powder

别名:

DNA 来源于小牛胸腺, 小牛胸腺DNA, 胸腺核酸

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关于此项目

化学文摘社编号:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
Biological source:
bovine pancreas
Form:
lyophilized powder
Solubility:
water: slightly soluble
Storage temp.:
−20°C
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biological source

bovine pancreas

Quality Level

grade

Molecular Biology

type

Type XV

form

lyophilized powder

solubility

water: slightly soluble

storage temp.

−20°C

General description

从雄性和雌性小牛的胸腺分离的高质量双链模板DNA。

Application

小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。
小牛胸腺DNA非常适合用于作为DNA聚合酶测定的底物、长片段的扩增、以及作为沉淀时的载体DNA。它被用作确定凝胶中DNA含量的标准品 以及通过荧光检测对DNA进行定量。

Features and Benefits

•在37°C温育16小时后未检测到核酸酶活性
•高质量的模板DNA

Preparation Note

1mg DNA约为20个 A260 单位。
使用Aposhian和Kornberg的方法,从高度聚合的小牛胸腺DNA中进行制备,并用DNase进行切口。
将DNA在分子生物级水中溶解过夜,终浓度为1mg/ml。


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Brian D Holt et al.
Advanced healthcare materials, 5(23), 3056-3066 (2016-12-08)
Synthetic biomaterials are poised to transform medicine; however, current synthetic options have yet to ideally recapitulate the desirable properties of native tissue. Thus, the development of new synthetic biomaterials remains an active challenge. Due to its excellent properties, including electrical
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)
Benjamin D Dickson et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-24)
Poly(ADP-ribose)polymerase (PARP) inhibitors (PARPi) have recently been approved for the treatment of breast and ovarian tumors with defects in homologous recombination repair (HRR). Although it has been demonstrated that PARPi also sensitize HRR competent tumors to cytotoxic chemotherapies or radiotherapy