Quality Level
usage
sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications
feature
dNTPs included, hotstart, Direct PCR
technique(s)
PCR: suitable
color
colorless
input
crude DNA
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
General description
Extract-N-Amp™ PCR ReadyMix™是一种专门配制的热启动PCR预混液，可直接以提取物进行PCR扩增。
PCR ReadyMix 预期与 Sigma 的 Extract-N-Amp Plant PCR 试剂盒和 Extract-N-Amp Tissue PCR 试剂盒配套使用。 所有 Extract-N-Amp 试剂盒均包括 PCR ReadyMix，每次提取可充分进行一次 PCR 反应。但是，如果需要额外的 PCR 反应，可能需要补充 PCR ReadyMix。
Application
Extract-N-Amp ™PCR ReadyMix™已被用于以下用途：
- 基因组 DNA 提取
- 真菌 DNA提取和聚合酶链反应（PCR）扩增
- 直接 PCR 扩增
- 基因分型
- 实时PCR
Legal Information
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
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Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
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Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex
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The objective of our study was the development of a semi-quantitative real-time PCR to detect uropathogens. Two multiplex PCR reactions were designed to detect Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter spp., Proteus mirabilis, Enterococcus faecalis, and Pseudomonas aeruginosa. 16S
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In Dutch laboratories molecular detection of B. pertussis and B. parapertussis is commonly based on insertion sequences IS481 and IS1001, respectively. Both IS elements are more widely spread among Bordetella species. Both Bordetella holmesii, and B. bronchiseptica can harbour IS481.