F9291
抗FLAG® BioM2单克隆抗体 小鼠抗
clone M2, purified immunoglobulin, buffered aqueous glycerol solution
别名:
单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
M2, monoclonal
Application:
DB
Citations:
128
Quality Level
biological source
mouse
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous glycerol solution
species reactivity
all
concentration
~1 mg/mL
technique(s)
dot blot: suitable (chemiluminescent detection)
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
FLAG BioM2小鼠单克隆抗体通过酰肼键与生物素共价连接。该抗体可识别FLAG融合蛋白N末端、Met-N末端或C末端的FLAG序列。
Application
生物素标记抗体被用于使用亲和素或链霉亲和素偶联报告酶（如链霉亲和素-过氧化物酶）的免疫检测方法。 当使用小鼠细胞作为重组蛋白宿主时，首选一抗偶联物。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。
请访问我们的 FLAG®应用 门户网站，了解更多产品详情。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。
请访问我们的 FLAG®应用 门户网站，了解更多产品详情。
Physical form
溶于50％甘油，10 mM磷酸钠，pH 7.25，含有150mM NaCl和0.02％叠氮化钠
Preparation Note
用TBS（0.05M Tris，pH7.4，含有0.15M NaCl）稀释抗体至终浓度为1-10μg/mL。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时，它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因，它们在细胞表面的表现通常被称为“糖”。
Yingru Liu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(6), 2025-2038 (2010-02-12)
To assess the effects and mechanisms of a CD200R1 agonist administered during the progressive stage of a multiple sclerosis model, we administered CD200R1 agonist (CD200Fc) or control IgG2a during the chronic phase of disease (days 10-30) in mice with experimental
Hong-Won Lee et al.
Nature communications, 4, 1505-1505 (2013-02-21)
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified
J Zhu et al.
Oncogene, 35(15), 1955-1964 (2015-07-07)
The atypical E3 ubiquitin ligase RNF31 is highly expressed in human breast cancer, the most frequent neoplastic lethality among women. Here, RNF31 depletion in breast cancer cells in combination with global gene expression profiling revealed p53 (TP53) signaling as a