产品名称
Iscove 改良杜氏培养基, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
L-glutamine: no
glucose: yes
shipped in
ambient
storage temp.
2-8°C
Application
Guilbert和Iscove证明红细胞和巨噬细胞的前体细胞可以在完全确定的无血清培养基中进行培养，该培养基需补充白蛋白、转铁蛋白、卵磷脂和硒。该培养基是Dulbecco′s改良Eagle′s培养基（DME）的改良版本，其含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES缓冲液、以及硝酸钾（代替硝酸铁）。进一步的研究表明，Iscove′s培养基可以支持小鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和各种杂交细胞的成长。
Preparation Note
以0.584 g/L补充 L-谷氨酰胺
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Dillon M Voss et al.
Cancer research, 80(21), 4681-4692 (2020-09-16)
Muscleblind-like proteins (MBNL) belong to a family of tissue-specific regulators of RNA metabolism that control premessenger RNA splicing. Inactivation of MBNL causes an adult-to-fetal alternative splicing transition, resulting in the development of myotonic dystrophy. We have previously shown that the
Sophie G Kellaway et al.
Haematologica, 106(6), 1569-1580 (2020-04-18)
Hematological malignancies are characterised by a block in differentiation, which in many cases is caused by recurrent mutations affecting the activity of hematopoietic transcription factors. RUNX1-EVI1 is a fusion protein formed by the t(3;21) translocation linking two transcription factors required
Kirsten Gillingwater
Parasitology, 145(7), 953-960 (2017-11-24)
Trypanosoma equiperdum is a protozoan parasite responsible for causing Dourine, a debilitating neglected veterinary disease, found worldwide affecting equids. It is the only pathogenic trypanosome species that does not require an invertebrate vector for transmission, thus being passed from animal