Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
洛斯维帕克纪念研究所培养基通常被称为PRMI。 是一种用于细胞培养和组织培养的培养基。该培养基含有磷酸，适用于含有5%二氧化碳的气体。它可用于人淋巴细胞生长。
Application
RPMI-1640培养基用于以下应用：
- 用于细胞培养基（即在补充其它成分的 RPMI 1640 培养基中培养鼠胸腺瘤 EL-4 细胞）
- 用于脾细胞的分枝杆菌感染（在不含抗生素的 RPMI 1640 培养基中）
- 用于细胞分离和培养
- 用于淋巴细胞分离和培养、ELISPOT分析、体外增殖检测的培养基
- 用于gp39 基因表达和 CD40-免疫球蛋白结合检测的培养基
- 用于制备抗真菌剂
Preparation Note
按照每升培养基10.4克粉末配制。
补充2.0 g/L碳酸氢钠。
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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