HPLC色谱柱

Supelco® HPLC 和 UHPLC 色谱柱可满足当今快速 U/HPLC、LC-MS 及生物聚合物分离的需求,同时也符合各行业药典及监管机构规定的分析方法要求。Supelco® HPLC 产品采用 Fused-Core® 颗粒技术、整体式二氧化硅以及全多孔颗粒(包括超纯二氧化硅)。 此外,还提供多孔石墨碳(PGC)颗粒和聚合物颗粒,以满足高pH值稳定性要求。
Products
多孔石墨碳(PGC)颗粒
多孔石墨碳(PGC)色谱柱(如 Supel™ 碳液相色谱柱)具有独特的色谱性能,这是硅胶填充色谱柱无法比拟的。该色谱柱不仅适用于整个 pH 范围,还能在高温(> 200 °C)下使用,为色谱工作者开辟了新的领域。
表面多孔二氧化硅颗粒(SPP)/Fused Core® 技术
与同尺寸的全多孔颗粒相比,表面多孔二氧化硅色谱柱具有更高的流速和效率。Ascentis® Express和BIOshell™等Fused-Core® HPLC色谱柱可将任何HPLC系统转变为具备最高流速和性能的高速HPLC主力设备。
整体式硅胶
- 基于整体式二氧化硅的 HPLC 色谱柱(如 Chromolith® HPLC 色谱柱)可在极低的色谱柱背压下实现快速分离,同时具有较高的基质耐受性及更长的色谱柱使用寿命,因此是用于高通量、高性价比分析基质丰富样品的理想产品。
- Chromolith® HPLC色谱柱由具有双峰孔隙结构的整体式二氧化硅制成,包含2 µm的大孔和13 nm的中孔。这种结构赋予了色谱柱多种关键优势。
- Chromolith® WP色谱柱是宽孔(300 Å)整体式二氧化硅色谱柱,由单根连续的高纯度多孔二氧化硅棒制成。与传统色谱柱相比,这些色谱柱表现出了巨大的潜力,在更长的使用寿命内性能始终如一。除了各种反相(RP)相外,还提供可进行客户定制改性的蛋白A亲和色谱柱和环氧树脂色谱柱。
全多孔二氧化硅颗粒(FPP)B型(高纯度二氧化硅)
- Purospher® STAR HPLC 和 UHPLC 色谱柱最适合对碱性、中性及金属螯合样品进行无峰尾的分离。其卓越的性能和稳定性,结合扩展的 pH 稳定性,为方法开发提供了最大的灵活性。
- Discovery® HPLC色谱柱可高效分析生物分子和小分子。这些色谱柱具有极性与非极性键合,在LC-MS应用中具备独特的保留、选择性和无渗出特性。
- Ascentis® 键合相具有广泛的选择性。B型二氧化硅使色谱柱具有惰性、稳定性和可重复性。二氧化硅的高比表面积使其适用于制备级加样容量。
- SeQuant® HILIC 高效液相色谱柱(亲水相互作用液相色谱)带有高密度键合的两性离子官能团。这些 HILIC 色谱柱提供多种长度、粒径和孔径规格,同时具有卓越的重复性和鲁棒性。
- Sepax® HPLC色谱柱是基于高孔隙率二氧化硅的色谱柱,主要用于肽和蛋白质的分析。
全多孔二氧化硅颗粒(FPP)A型(常规二氧化硅)
- Supelcosil® 硅胶基 HPLC 色谱柱系列具有广泛的键合相化学结构,颗粒尺寸和色谱柱配置范围从微内径到制备级。这些色谱柱对极性、非极性和带电分析物均表现出极高的分离效率。
- LiChrospher® 和 Superspher® 高效液相色谱柱用途广泛且可靠,已在数千种成熟的 HPLC 方法中得到验证。
- LiChrosorb® 高效液相色谱柱采用不规则二氧化硅颗粒填充,粒径范围从分析级 5 µm 到适用于生产规模的大颗粒。
- TSKgel® HPLC 和 UHPLC 色谱柱适用于凝胶过滤、凝胶渗透、离子交换、反相、疏水作用、亲水作用及亲和色谱。(仅在美国和欧洲有售)
全多孔聚合物颗粒
- apHera™ 反相和氨基 HPLC 色谱柱在 pH 2-12 范围内稳定,且可使用碱性溶液进行清洗。它们是分析肽、小分子蛋白、碳水化合物、极性药物和有机酸的理想选择。
- Supelcogel® HPLC色谱柱采用聚苯乙烯-二乙烯基苯共聚物(PS-DVB)填充,因此具有良好的化学稳定性。两种不同交联度的PS-DVB共聚物(6%和8%交联)可最大限度地优化样品的分离过程。
- Hamilton 聚合物反相 HPLC 色谱柱具有极高的惰性、高效性和耐用性,且机械和热稳定性极佳。
- TSKgel® HPLC 和 UHPLC 色谱柱可用于分离蛋白质、肽及其他生物分子。这些色谱柱含有孔径各异的二氧化硅和甲基丙烯酸酯颗粒,可适应分子量范围广泛的分析物。(仅在美国和欧洲有售)
手性HPLC色谱柱
我们提供种类繁多的手性 HPLC 色谱柱,适用于各种应用。
- Astec CYCLOBOND™ 手性色谱柱采用键合环糊精相,可对有机分子进行选择性化学分离。
- Astec CHIROBIOTIC™ 手性色谱柱基于与高纯度硅胶结合的大环糖蛋白,适用于液相色谱-质谱(LC-MS)手性分析。
- Astec Cellulose DMP 手性色谱柱采用球形高纯度多孔二氧化硅,表面涂覆 DMPC(3,5-二甲基苯基氨基甲酸酯);可在正相、极性有机溶剂和超临界流体色谱(SFC)条件下分离广泛的手性化合物。
- Astec 铜配体交换手性色谱柱适用于分离羟基酸、氨基酸、双官能团外消旋体及其他胺类化合物。
相关资源
- Flyer: Purospher® STAR RP-18 endcapped, 2 µm Capillary Columns
Best in Class C18 phase for excellent peak symmetry, performance and pH stability.
- Flyer: SeQuant® ZIC®-HILIC and ZIC®-cHILIC Capillary Columns
SeQuant® ZIC®-HILIC and ZIC®-cHILIC columns are high-performance tools designed for separating polar hydrophilic compounds under hydrophilic interaction liquid chromatography (HILIC) conditions.
- Flyer: BIOshell™ A120 (2.7 µm) Oligo C18 INERTProve™ HPLC Columns: from 1.5 mm I.D. to 4.6 mm I.D.
Save time and solvent with rapid separations using BIOshell™ Oligo columns, designed for high efficiency and exceptional stability.
- Application Note: LC-MS/MS Analysis of Liraglutide on BIOshell™ A160 Peptide PCS-C18
We present an LC-MS/MS methodology utilizing the BIOshell™ A160 Peptide PCS-C18 column for characterizing liraglutide samples, which successfully identifies multiple impurities, including an N-terminal cyclization that can occur even when the compound is handled under mild conditions.
- Flyer: Ascentis® Express 160Å ZIC®-cHILIC Columns
The Ascentis® Express ZIC®-cHILIC column is designed for the effective retention and separation of polar and hydrophilic compounds, delivering robust chromatography with high selectivity and reproducibility.
- Flyer: Ascentis® Express 120 Å C18 pH+ U/HPLC Columns
The Ascentis® Express 120 Å C18 pH+ U/HPLC column is built on the reliable Fused-Core® particle technology, enhancing speed and efficiency. It features surface-modified organo-silane technology that provides alkaline resistance, ensuring excellent stability in high pH environments.
- /deepweb/assets/sigmaaldrich/marketing/global/documents/660/486/trypsin-cleaved-proteins-capillary-columns-wp14380en-ms.pdf
This study employs a Two-Dimensional Liquid Chromatography (2D-LC) approach, utilizing both Reversed-Phase Liquid Chromatography (RPLC) and Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC®-HILIC), combined with Time-of-Flight Mass Spectrometry (TOF-MS) detection, to analyze trypsin-cleaved proteins including Bovine Serum Albumin (BSA), cytochrome C, and ovalbumin.
- Flyer: Purospher® STAR RP-18 endcapped, 2 µm Capillary Columns
Best in Class C18 phase for excellent peak symmetry, performance and pH stability.
- Flyer: Ascentis® Express PCS Phenyl-Hexyl, 2.7 µm U/HPLC Columns
The Ascentis® Express 90 Å PCS Phenyl-Hexyl 2.7 µm U/HPLC column offers an alternative selectivity in comparison to C18 columns including PCS-C18 providing benefits for the separation of basic aromatic compounds with improved peak shape.
- Flyer: Ascentis®Express PCS-C18, 2.7 µm U/HPLC Columns
The Ascentis® Express PCS-C18 columns are designed for effective separation of basic, acidic, or neutral compounds using low ionic strength (formic acid) mobile phase conditions.
- Flyer: SeQuant® ZIC®-HILIC and ZIC®-cHILIC Capillary Columns
SeQuant® ZIC®-HILIC and ZIC®-cHILIC columns are high-performance tools designed for separating polar hydrophilic compounds under hydrophilic interaction liquid chromatography (HILIC) conditions.
- Brochure: HPLC Column Selection Guide
The comprehensive Supelco® portfolio of analytical solutions to meet your U/HPLC and LC-MS needs.
- Brochure: HPLC Method Development Guide
High performance liquid chromatography (HPLC) is a technique for separating analytes dissolved in a liquid, mobile phase by using their specific interaction with a stationary phase (chromatography column).
- Brochure: Ascentis® Express Columns
Based on Fused-Core® particle technology, Ascentis® Express columns provide a exceptional advancements in HPLC column performance and the benefits of high sample throughput at maximum resolution.
- Brochure: BIOshell™ Columns
The BIOshell™ line of superficially porous particle (SPP) packed columns has been developed to aid research into understanding the subtleties of the molecule that is being developed.
- Brochure: Chromolith® HPLC Columns
To truly accelerate chromatographic separations, there is no better choice than Chromolith® HPLC columns. Due to their revolutionary, monolithic technology, Chromolith® columns provide excellent and rapid separations with extremely high robustness and matrix tolerance compared to particulate columns.
- Brochure: Purospher STAR Columns
The success of any method depends on the quality of the stationary phase. Precise, long-term reproducibility is a key factor in achieving reliable results. The base silica of Purospher™ STAR columns is 99.999% pure.
- Brochure: Chiral HPLC Columns
Columns for versatile and robust Chiral HPLC and LC-MS separations.
- Brochure: HPLC Accessories
The use of HPLC systems to achieve faster analyses and increased resolution pushes systems toward higher operating pressures. For instance, faster flow rates, longer column lengths, and reduced particle sizes gain a performance advantage in HPLC, but at a cost of increased operating pressures.
- Brochure: Discovery Bio Columns
Many of the challenges facing researchers in the proteomics and biopharmaceutical fields are related to the need to obtain as much information as possible on very limited samples. We designed Discovery® BIO Wide Pore reversed-phase HPLC columns to address these challenges.
- Brochure: LC-MS Resource Guide
In this guide, we provide a premier selection of proven Supelco® tools and consumables that meet the requirements of scientists who primarily use the LC-MS technique for analysis of drugs, biomolecules separation and analytical assays.
- Brochure: LC-MS Contaminants
In this technical bulletin, you will learn some tips on identifying LC-MS contaminants and avoiding contamination. Our 110 years of separation expertise, combined with our precision-manufactured products, give you the greatest chance of obtaining reproducible, clean data.
- Application Note: Supelco® HPLC Products: The Best Choice for Any LC Instrument
Since the 1970’s, Supelco® has been a trusted name in high performance liquid chromatography (HPLC) consumables. From a diverse array of columns packed with different stationary phases to a complete portfolio of HPLC accessories, the Supelco® portfolio of HPLC consumables caters to the needs of all analysts.
- Application Guide: Drug of Abuse
Application compilation with focus on the LC-MS analysis of drugs of abuse in biological samples.
- HPLC Method Transfer Calculator
Easily transfer a method between HPLC columns, (scale between microbore through preparative range), based on the 2 column dimensions and current method conditions.
- Wall Chart: HPLC Packings for USP Compendial Methods
The official pharmaceutical analysis monographs in the United States Pharmacopeia (USP) detail the methods used by pharmaceutical manufacturers for quality control of bulk drug substances and dosage form preparations.
- Application Guide: Ascentis® Express and BIOshell™ Column Cleaning Procedures and Best Practices
To maximize the lifetime of an HPLC column and ensure its top performance, it is necessary to properly maintain the column. Many different factors can contribute to loss of resolution or sensitivity, but often times the following factors lead to the vast majority of column performance issues.
- Flyer: Supel™ Carbon LC Capillary UHPLC Columns
Superior separation of challenging compounds at the capillary scale.
- White Paper: Retention Behavior of Mono- and Disaccharides on a Supel™ Carbon LC column
PGC LC-MS-based profiling of mono- and disaccharides.
教程
了解如何将HPLC色谱柱和滤芯与相应的预柱、固定座和保护柱连接起来。
*东曹生物科学色谱柱在以下国家有售:
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