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PC78 Anti-Fas Ligand (Ab-1) (261-277) Rabbit pAb

Anti-Fas Ligand (Ab-1) (261-277) Rabbit pAb: Malzeme Güvenlik Bilgi Formu (MSDS) veya SDS, Analiz Sertifikası (COA) ve Kalite Uygunluk Sertifikası (COQ), dosyalar, broşürler ve diğer dokümanlar.

Synonyms: Anti-CD178, Anti-CD95L

PC78
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~35-53 kDa Fas ligand in induced HuT78 and THP.1 cells.
      Catalogue NumberPC78
      Brand Family Calbiochem®
      SynonymsAnti-CD178, Anti-CD95L
      References
      ReferencesBrunner, T., et al. 1995. Nature 373, 441.
      Ju, S-T., et al. 1995. Nature 374, 444.
      Hughes, D.P.M. and Crispe, I.N. 1995. M. Exp. Med. 182, 1395.
      Nagata, S. and Golstein, P. 1995. Science 267, 1449.
      Tanaka, M., et al. 1995. EMBO J. 14, 1129.
      Hanabuchi, S., et al. 1994. Proc. Natl. Acad. Sci. 91, 4930.
      Takahashi, T., et al. 1994. Int. Immunol. 6, 1567.
      Rouvier, E., et al. 1993. J. Exp. Med. 177,195.
      Suda, T., et al. 1993. Cell 75, 1169.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlInduced Hut78 or THP.1 cells or mouse PBL
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Not Frozen Sections
      Not Immunofluorescence
      Not Immunoprecipitation
      Not Paraffin Sections
      Application NotesImmunoblotting (5 µg/ml)
      Frozen Sections (not recommended)
      Immunofluoresence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      Application CommentsFor positive control, induce Fas ligand by incubating overnight in the presence of 5 µg/ml ConA. Co=incubation with 1 ng/ml IL-2 will induce higher levels. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (QLSLINFEESKTFFGL) corresponding to amino acids 261-277 of mouse Fas ligand
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC78 0

      Documentation

      Anti-Fas Ligand (Ab-1) (261-277) Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Fas Ligand (Ab-1) (261-277) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC78

      References

      Reference overview
      Brunner, T., et al. 1995. Nature 373, 441.
      Ju, S-T., et al. 1995. Nature 374, 444.
      Hughes, D.P.M. and Crispe, I.N. 1995. M. Exp. Med. 182, 1395.
      Nagata, S. and Golstein, P. 1995. Science 267, 1449.
      Tanaka, M., et al. 1995. EMBO J. 14, 1129.
      Hanabuchi, S., et al. 1994. Proc. Natl. Acad. Sci. 91, 4930.
      Takahashi, T., et al. 1994. Int. Immunol. 6, 1567.
      Rouvier, E., et al. 1993. J. Exp. Med. 177,195.
      Suda, T., et al. 1993. Cell 75, 1169.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      SynonymsAnti-CD178, Anti-CD95L
      ApplicationImmunoblotting (5 µg/ml)
      Frozen Sections (not recommended)
      Immunofluoresence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      DescriptionPurified rabbit polyclonal antibody. Recognizes Fas ligand.
      BackgroundThe ligand for fas/APO-1/CD95 was identified in a cytotoxic T cell hybridoma that could lyse cells expressing fas/APO-1 but not cells lacking this receptor. Subsequent work directly demonstrated that triggering through the fas molecule by the fas ligand was responsible for the induction of apoptosis. The fas ligand is a member of the TNF family of proteins and is classified as a type II membrane protein. The highest degree of homology with TNF occurs in the extracellular domain which corresponds to the COOH terminal portion of the fas protein. The fas ligand has four potential N-glycosylation sites which appear to be variably used. Because of the variation in glycosylation, the full-length protein migrates as several distinct molecular weight species by SDS-PAGE. In addition to the full-length protein of ~35-43 kDa, it has been shown that the fas ligand is also cleaved from the surface of the cell and released as a soluble form approximately 26 kDa in size. There is still debate as to whether the protein is actively cleaved or is simply released/shed from the cell membrane. The chromosomal location of fas ligand has been mapped to 1q23 and the gene is split into 4 exons spanning a distance of about 8.0 kb. Involvement of fas/fas ligand has now been demonstrated as being a general mechanism for the action of cytotoxic T lymphocytes.
      HostRabbit
      Immunogen speciesMouse
      Immunogena synthetic peptide (QLSLINFEESKTFFGL) corresponding to amino acids 261-277 of mouse Fas ligand
      IsotypeIgG
      Specieshuman, mouse
      Positive controlInduced Hut78 or THP.1 cells or mouse PBL
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor positive control, induce Fas ligand by incubating overnight in the presence of 5 µg/ml ConA. Co=incubation with 1 ng/ml IL-2 will induce higher levels. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBrunner, T., et al. 1995. Nature 373, 441.
      Ju, S-T., et al. 1995. Nature 374, 444.
      Hughes, D.P.M. and Crispe, I.N. 1995. M. Exp. Med. 182, 1395.
      Nagata, S. and Golstein, P. 1995. Science 267, 1449.
      Tanaka, M., et al. 1995. EMBO J. 14, 1129.
      Hanabuchi, S., et al. 1994. Proc. Natl. Acad. Sci. 91, 4930.
      Takahashi, T., et al. 1994. Int. Immunol. 6, 1567.
      Rouvier, E., et al. 1993. J. Exp. Med. 177,195.
      Suda, T., et al. 1993. Cell 75, 1169.