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CA1039 Anti-HSPA1B Mouse mAb (3B7)

Anti-HSPA1B Mouse mAb (3B7): Malzeme Güvenlik Bilgi Formu (MSDS) veya SDS, Analiz Sertifikası (COA) ve Kalite Uygunluk Sertifikası (COQ), dosyalar, broşürler ve diğer dokümanlar.

Synonyms: Anti-Heat Shock Protein 70 1B

CA1039
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~70 kDa HSPA1B protein in HeLa, PC-12, IMR-32, Raw 264.7, and NIH3T3 cells and human testis tissue.
      Catalogue NumberCA1039
      Brand Family Calbiochem®
      SynonymsAnti-Heat Shock Protein 70 1B
      Application Data
      Detection of rat HSPA1B by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HSPA1B by immunoblotting. Sample: Whole cell lysate from IMR-32 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse HSPA1B by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HSPA1B by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (6 µg/ml). Detection: colorimetric (DAB).

      Detection of human HSPA1B by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlPC12 cells, IMR-32 cells, Raw 264.7 cells, NIH3T3 cells, Human testis tissue, HeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (6 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 531-612 of human HSPA1B, expressed as a GST fusion protein
      Clone3B7
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CA1039 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-Heat Shock Protein 70 1B
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (6 µg/ml)
      Application Data
      Detection of rat HSPA1B by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HSPA1B by immunoblotting. Sample: Whole cell lysate from IMR-32 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse HSPA1B by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human HSPA1B by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (6 µg/ml). Detection: colorimetric (DAB).

      Detection of human HSPA1B by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (10 µg/ml). Detection: fluorescence.
      DescriptionA purified mouse monoclonal antibody that recognizes the ~70 kDa HSPA1B protein.
      BackgroundHSPA1B is a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 531-612 of human HSPA1B, expressed as a GST fusion protein
      Clone3B7
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlPC12 cells, IMR-32 cells, Raw 264.7 cells, NIH3T3 cells, Human testis tissue, HeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review