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  • Should MultiScreen MAHA and MSHA ELISpot plates with MCE membranes be initially pre-wet with alcoho…

    No. Neither is necessary. Methanol will dissolve the mixed-cellulose ester membrane. PBS is no longer needed. Membrane improvements ensure instant wetting of the membrane.
    Document Type:
    FAQ
    Product Catalog Number:
    C9039
    Product Catalog Name:
    Elispot MultiScreen®HTS Filter Plates and Antibody Pairs

  • When performing an ELISpot assay should MultiScreen plates with Immobilon-P membrane (MAIP and MSIP)…

    Yes. Wetting the membrane with 15 µL alcohol followed by two to three washes with sterile PBS will allow the antibody more intimate contact with the membrane. Do not vacuum the alcohol through the underdrain, rather it should be flicked out. Once the membrane is pre-wet with alcohol, do not allow it to dry for the duration of the assay.
    Document Type:
    FAQ
    Product Catalog Number:
    C9039
    Product Catalog Name:
    Elispot MultiScreen®HTS Filter Plates and Antibody Pairs

  • We are looking to coat MultiScreen ELISpot plates with primary antibody and store them. Do you have …

    After coating with primary antibody overnight at 4°C, wash twice with 150µL sterile Milli-Q® water. Dry plates thoroughly under sterile conditions and store for up to one week at 4°C. If longer storage is desired, the plates should be washed as previously described and then blocked (150µL/well media, 37°C, 2hr). Following blocking, wash 2x with 150µL sterile Milli-Q. Dry as before and store desiccated. Ab activity may diminish over time - especially in the event that the plates have not been blocked. Millipore has not determined shelf-life for either protocol as performance stability is likely to depend largely on the properties of the specific antibody under investigation.
    Document Type:
    FAQ
    Product Catalog Number:
    C9039
    Product Catalog Name:
    Elispot MultiScreen®HTS Filter Plates and Antibody Pairs