Poster: Multiplex Magnetic Bead Assay Panel for Porcine Cytokine/Chemokine Biomarkers Using Luminex®xMAP®Technology
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Multiplex Assays to Detect Antibodies which Recognize SARS-CoV-2 Antigenic Proteins in Human Serum and Plasma
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- Technical Information
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Multiplex pluripotency analysis of STEMCCA™ vector-reprogrammed iPS cells using MILLIPLEX®map magnetic bead assays
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- Technical Information
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Custom-designed MLPA using multiple short synthetic probes: application to methylation analysis of five promoter CpG islands in tumor and urine specimens from patients wi ...
- Ligation of two oligonucleotide probes hybridized adjacently to a DNA template has been widely used for detection of genome alterations. The multiplex ligation-dependent probe amplification (MLPA) technique allows simultaneous screening of multiple target sequences in a single reaction by using pairs of probes that carry tails for binding of common amplification primers. Resolution of the various targets is achieved by electrophoresis on the basis of predefined differences in amplicon length. In the conventional MLPA approach, one of the two target probes is generated by cloning in a single-stranded bacteriophage vector to introduce a sequence of defined length between the primer binding site and the specific target sequence. Here we demonstrate that differences in amplicon length can be achieved by using multiple short synthetic probes for each target sequence. When joined by a DNA ligase, these probes will form a single amplifiable template whose length is defined by the number and lengths of the individual probes. We have used this principle to establish a methylation-specific MLPA (MS-MLPA) assay that simultaneously determines the methylation status of five promoter CpG islands, and we have used this assay to analyze DNA from tumor tissue and corresponding urine samples from patients with bladder cancer. Our data show that the use of multiple short synthetic probes provides a simple means for custom-designed MS-MLPA analysis.
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- Reference
- Product Catalog Number:
- 2100
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- Protein-Concentrate Kit (Micro)
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Multiplex Western blotting system for the analysis of muscular dystrophy proteins
- Document Type:
- Reference
- Product Catalog Number:
- MAB1922
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- Anti-Laminin α2 Antibody, clone 5H2
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Multiplex Analysis Approach to Discover Plasma-based, Progression Subtype-specific Biomarker Candidates for Multiple Sclerosis
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Multiplex Detection of Stem Cell Pluripotency Markers in Reference Cancer Cell Lines
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Multiplex Biomarker Panels for Determining Drug-Induced Vascular Injury (DIVI) in Rat
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Multiplex Detection of the Stem Cell Pluripotency Markers c-Myc, Nanog, Oct 3/4 and Sox2: MILLIPLEX®MAP Human Stem Cell Pluripotency Magnetic Bead Panel 1
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Development of a cluster of differentiation antibody-based protein microarray.
- Protein microarrays combine aspects of DNA microarrays and ELISA for the parallel interrogation of a biological sample using a multiplex of protein biomarkers. Here we report the development of a protein microarray consisting of a subset of CD antibodies and CRP. Several preparations (culture supernatant, ascites fluid and purified Ig) of each antibody were used in a forward phase protein microarray. Microarrays were fabricated using a non-contact printer delivering 300 pL (+/-30 pL) to specific locations on polyacrylamide gel-based substrates. Following production, microarrays were blocked for non-specific binding and incubated with sera conjugated directly with Cy3. Using CRP as a control biomarker, 12 clinical samples (inflammatory conditions and controls) were interrogated using the protein microarray format and results compared to CRP measured by conventional immunoassay. The data obtained from the microarray correlated with CRP assessed by immunoassay. Subsequently CRP 'positive' samples were interrogated for CD antigen expression; which revealed CD25 and CD45RO expression in all samples. Whilst this study focussed on a subset of CD antibodies, it is anticipated that this array could be expanded to include a larger number of CD antibodies and allow screening of sera from multiple conditions in order to identify disease markers.
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- Multiple
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