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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

96-Well Plasmid and BAC Preparation Kit

Simply clear, concentrate, wash, and recover More

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Overview

Specifications

Ordering Information

96-Well Plasmid and BAC Preparation KitClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pack Size
LSKP09604	Plasmid Miniprep₉₆ Kit	4
LSKP09624	Plasmid Miniprep₉₆ Kit	24

Required EquimentClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pack Size
MSVMHTS00	MultiScreen_HTS Vacuum Manifold	1
WP6111560	Chemical Duty Pump, 115 V/60 Hz	1
WP6122050	Chemical Duty Pump, 220 V/50 Hz	1
WP6110060	Chemical Duty Pump, 100 V/50–60 Hz	1

Reagents*Clear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Volume
LSKCRS500	Cell Resuspension Solution 0.5 L	500 mL
LSKCLS500	Cell Lysis Solution 0.5 L	500 mL
LSKNS0500	Neutralization Solution, 0.5 L	500 mL
LSKNF0500	Plasmid Wash Solution	500 mL
LSKCTB500	Tris Buffer, 0.5 L	500 mL
LSKPMRN30	RNase A, 0.9 mL	0.9 mL

*All reagents are supplied 1 bottle/pack

Documentation

Related Products & Applications

Categories

Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > Plasmid Purification Kits & Reagents

Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates

C7481Simply clear, concentrate, wash, and recover

The Montage Plasmid Miniprep_HTS 96 Kit is a fast, easy-to-use kit for high-purity plasmid or bacterial artificial chromosome (BAC) minipreps. Using a patented separation technology, the Montage Kit follows a simple protocol that eliminates lengthy bind/elute methods and centrifugation steps to yield clean and reproducible DNA in 50% less time than traditional methods.

Purified PCR samples are suitable for the most sensitive downstream applications including cloning, DNA sequencing, and transformation. BAC applications include sequencing, fingerprinting, arraying, and PCR amplification.

The kit includes all the reagents and disposable materials you need to purify plasmid or BAC DNA in a 96-well format. Following bacterial lysis, three short filtration steps are all that are required to prepare 96 clean DNA samples from each plate.

The Montage Plasmid Miniprep_HTS 96 Kit is ideal for use with automated liquid handlers. Collection of purified sample from the top of the plate and the elimination of lengthy centrifugation steps enhance the kit's compatibility with a wide range of automated systems.

No centrifugation or precipitation steps required
Minimum processing time
Excellent purity, yields and reproducibility
Automation compatible

Kit Components

Part	Function
96-well deep well culture block	Growth of host bacteria
Plasmid plate	Purification of plasmid DNA
Lysate clearing plate	Clearing of bacterial lysate
V-bottom storage plate	Storage of plasmid DNA samples
Sealing tape	Sealing of V-bottom storage plate
RNase A	Required additive for Solution 1
Solution 1	Cell resuspension
Solution 2	Cell lysis
Solution 3	Neutralization
Solution 4	Wash
Solution 5	Resuspension/storage of plasmid DNA
Foil seal	Sealing plates during growth (must be pierced)
For more information on kit components, refer to the Montage Plasmid Miniprep_HTS 96 Kit operation manual or visit www.millipore.com/montage.

Protocol for Plasmid DNA Miniprep

For BAC DNA protocol, see Montage Plasmid Miniprep_HTS Kit user guide, P36315

1.	Following bacterial lysis, transfer lysates from deep well culture block into clearing plate. Clear lysates into Plasmid filter plate using vacuum.
2.	Move Plasmid plate to top of manifold and apply vacuum for 5 to 7 minutes to concentrate sample. Plasmid DNA is retained on the membrane surface while contaminants are filtered to waste. Add wash solution and filter for 3 to 5 minutes.
3.	Add resuspension buffer and collect purified sample by aspiration.
4.	Transfer samples to V-bottom plate for use or storage.

Performance — Plasmid DNA

High Quality

Agarose gel showing quality and consistency of plasmid DNA purified using the Montage Plasmid Miniprep<sub>HTS</sub> 96 Kit (MW-lambda <i>Hin</i>d III digest).

Agarose gel showing quality and consistency of plasmid DNA purified using the Montage Plasmid Miniprep_HTS 96 Kit (MW-lambda Hind III digest).

High Yields*
Host strain	Plasmid	Yield (µg)
XL 1-blue	pUC19	2.1 – 6.1
XL 1-blue	pLH2**	3.5 – 9.2
JM109	pUC19	2.4 – 6.4
JM109	pLH2**	3.5 – 9.1
Results will vary depending on host strain, plasmid, and protocol conditions. pLH2 was derived from pUC19. The 2.0 Kb fragment of Hind III digested l phage was cloned into the Hin*d III site of pUC19, resulting in a 4.7 Kb plasmid.

Reliable Results
Host strain	Plasmid	Average Read Length (n=24; >98% accuracy)	Average Phred q >20 bases (n = 24)
XL1-blue	pUC19	707	618
XL1-blue	pLH2	658	623
JM109	pUC19	706	602
JM109	pLH2	695	543
Sequence data from plasmids prepared with the Montage Plasmid Miniprep_HTS 96 Kit. All samples were sequenced using BigDye sequencing chemistry and were run on a MegaBACE® capillary array sequencer. 160 ng of pLH2 and 100 ng of pUC19 were used in the respective reactions. Reactions were cleaned up using Montage SEQ₃₈₄ Kit.

Performance— BAC DNA

Chromosomal DNA

DNA from three different BAC clones from a human chromosome 22 human BAC DNA library (Research Genetics, Inc.) was purified with the Montage Plasmid Miniprep_HTS 96 Kit and electrophoresed on a 0.8% agarose gel for 120 min at 100 V either uncut (lanes 1 – 3) or after digestion with Not I (lanes 4 – 6) or Hind III (lanes 7 – 9). Lane M is a Hind III digest of Lambda phage DNA. The BAC DNA purified with the Montage Plasmid Miniprep_HTS 96 Kit is suitable for fingerprinting.

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