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MAB3804 Anti-53BP1 Antibody, clone BP18

Anti-53BP1 Antibody, clone BP18 is a Mouse Monoclonal Antibody for detection of 53BP1 also known as p53 Binding Protein 1 & has been validated in IF & WB.

Anti-53BP1 Antibody, clone BP18 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: TP53BP1, 53BP1, MGC138366, p202, p53BP1, FLJ41424 UniProt Number: Q12888
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MAB3804
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HIF, WBMAscitesMonoclonal Antibody
Description
Catalogue NumberMAB3804
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-53BP1 Antibody, clone BP18
Alternate Names
  • p53 Binding Protein 1
References
Product Information
FormatAscites
PresentationAscites. Liquid.
Quality LevelMQ100
Applications
ApplicationAnti-53BP1 Antibody, clone BP18 is a Mouse Monoclonal Antibody for detection of 53BP1 also known as p53 Binding Protein 1 & has been validated in IF & WB.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesWestern blot. Recognizes a protein of >250 kD. Suggested dilution buffer is TBST. Suggested blocking buffer is 5% non-fat dry milk.

Immunocytochemistry

Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenRecombinant human 53BP1 proteins.
CloneBP18
HostMouse
Specificity53BP1
IsotypeIgM
Species Reactivity
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TP53BP1
  • 53BP1
  • MGC138366
  • p202
  • p53BP1
  • FLJ41424
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q12888 # May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Binds to sites of DNA damage. Plays a role in the response to DNA damage.
SIZE: 1972 amino acids; 213574 Da
SUBUNIT: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H3 that has been methylated on 'Lys-79'. Does not bind unmethylated histone H3.
SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks.
PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. & Phosphorylated in an ATM-dependent manner in response to DNA damage.
SIMILARITY: SwissProt: Q12888 ## Contains 2 BRCT domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3804 04053252466168

Documentation

Anti-53BP1 Antibody, clone BP18 MSDS

Title

Safety Data Sheet (SDS) 

Anti-53BP1 Antibody, clone BP18 Certificates of Analysis

TitleLot Number
MOUSE ANTI-53BP1 MONOCLONAL ANTIBODY - 2388959 2388959
MOUSE ANTI-53BP1 - 3202259 3202259
MOUSE ANTI-53BP1 - 3407306 3407306
MOUSE ANTI-53BP1 - 3587223 3587223
MOUSE ANTI-53BP1 - 3792957 3792957
MOUSE ANTI-53BP1 - 4041933 4041933
MOUSE ANTI-53BP1 -2796318 2796318
MOUSE ANTI-53BP1 -2844572 2844572
MOUSE ANTI-53BP1 MONOCLONAL ANTIBODY 2938838
MOUSE ANTI-53BP1 MONOCLONAL ANTIBODY - 1999450 1999450
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