Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABT1485-AF647 Anti-Actin Antibody, Alexa Fluor® 647 Conjugate

This rabbit polyclonal Anti-Actin, Alexa Fluor® 647 Conjugate, Cat. No. ABT1485-AF647 s validated for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence applications for the detection of Actin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ACTA1, ACTA, CFTD, NEM3, MPFD UniProt Number: P68133
View Products on Sigmaaldrich.com
ABT1485-AF647
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HFC, ICC, IFRbAlexaFluor®647Polyclonal Antibody
Description
Catalogue NumberABT1485-AF647
DescriptionAnti-Actin Antibody, Alexa Fluor® 647 Conjugate
Alternate Names
  • Actin
  • alpha skeletal muscle
  • Alpha-actin-1
Background InformationActins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.
References
Product Information
FormatAlexaFluor®647
PresentationPurified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rabbit polyclonal Anti-Actin, Alexa Fluor® 647 Conjugate, Cat. No. ABT1485-AF647 s validated for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence applications for the detection of Actin.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
Application NotesFlow Cytometry Analysis: 2 µL from a representative lot detected actin in one million HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in NIH/3T3 cells.

Immunofluorescence Analysis: A 1:50-150 dilution from a representative lot detected actin in human heart, prostate, skeletal muscle, and small intestine tissue sections.

The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor® 488-conjugated antibody (Cat. No. ABT1485-AF488) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis Alexa Fluor® 647 conjugate was prepared from purified rabbit polyclonal antibody (Cat. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse. Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ACTA1
  • ACTA
  • CFTD
  • NEM3
  • MPFD
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~42 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT1485-AF647 04054839083648