Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN376 Anti-Alanyl-tRNA synthetase (AARS Antibody), clone M6-P2E5

This Anti-Alanyl-tRNA synthetase (AARS Antibody), clone M6-P2E5 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Alanyl-tRNA synthetase (AARS).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: AARS UniProt Number: P49588
MABN376
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN376
DescriptionAnti-Alanyl-tRNA synthetase (AARS Antibody), clone M6-P2E5
Alternate Names
  • Alanine-tRNA ligase, cytoplasmic
  • Alanyl-tRNA synthetase
  • AlaRS
  • Renal carcinoma antigen NY-REN-42
Background InformationAlanyl-tRNA synthetase (AARS), also known as Alanine-tRNA ligase, AlaRS, or Renal carcinoma antigen NY-REN-42, and encoded by the gene AARS, is a key transfer RNA synthetase responsible for the attachment of alanine to the tRNA(Ala) for protein translation. Alanine-tRNA ligase also can edit incorrectly charged tRNA(Ala) for proper translation. Alanine-tRNA ligase is a monomer and is localized in the cytoplasm and ubiquitously expressed. Mutations in Alanine-tRNA ligase have been linked to a progressive peripheral nervous disorder called Charcot-Marie Tooth disease. Moreover autoantibodies against aminoacyl tRNA synthetases are some of the most common autoantibodies detected in autoimmune diseases with nearly 25-35% of patients having them, and some of these such as autoantibodies to Alanine-tRNA ligase have also been associated with autologous antibodies in renal carcinoma patients.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Alanyl-tRNA synthetase (AARS Antibody), clone M6-P2E5 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Alanyl-tRNA synthetase (AARS).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected Alanyl-tRNA synthetase (AARS) in 10 µg of HepG2 cell lysate.
Immunohistochemistry Analysis: A representative lot detected Alanyl-tRNA synthetase (AARS) in human breast cancer tissue (Prof. Ayham Alnabulsi, University of Aberdeen)
Biological Information
ImmunogenLinear peptide corresponding to the C-terminus of human Alanyl-tRNA synthetase (AARS).
EpitopeC-terminus
CloneP2E5
Concentration1 mg/mL
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • AARS
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~110 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Alanyl-tRNA synthetase (AARS) in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN376 04053252978791

Related Products & Applications