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MABF459 Anti-CD86 (B7-2) Antibody (human), PE, clone IT2.2

This mouse monoclonal antibody Anti-CD86 (B7-2) (human), PE, clone IT2.2 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD86 (B7-2).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD86, CD28LG2 UniProt Number: P42081
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MABF459
100 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, IP, FCMPEMonoclonal Antibody
Description
Catalogue NumberMABF459
DescriptionAnti-CD86 (B7-2) Antibody (human), PE, clone IT2.2
Alternate Names
  • T-lymphocyte activation antigen CD86
  • Activation B7-2 antigen
  • B70
  • BU63
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • CD antigen CD86
Background InformationThe IT2.2 antibody reacts with human CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.The IT2.2 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells. It is reported to be cross-reactive with Rhesus, Cynomolgus and Common marmoset CD86.
References
Product Information
FormatPE
PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal antibody Anti-CD86 (B7-2) (human), PE, clone IT2.2 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD86 (B7-2).
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneIT2.2
Concentration5 µL (0.5 µg)/test
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CD86
  • CD28LG2
Purification MethodSize Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD86 (B7-2) in one million human peripheral blood monocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF459 04053252960482