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MABD42 Anti-Cyp7a1 Antibody, clone 15B9.1

This Cyp7a1 antibody is validated for use in WB & IHC for the detection of the Cyp7a1 protein.

Anti-Cyp7a1 Antibody, clone 15B9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CYP7A1, CYP7 UniProt Number: P22680
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MABD42
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABD42
DescriptionAnti-Cyp7a1 Antibody, clone 15B9.1
Alternate Names
  • Cholesterol 7-alpha-monooxygenase
  • CYPVII
  • Cholesterol 7-alpha-hydroxylase
  • Cytochrome P450 7A1
Background InformationCytochrome P450 7A1 (CYP7A1, CYPVII), also known as cholesterol 7-alpha-monooxygenase or cholesterol 7-alpha-hydroxylase, belongs to the cytochrome P450 family and is important for cholesterol homeostasis. CYP7A1 catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Detected primarily in the liver, CYP7A1 catalyzes the following reaction: Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O. CYP7A1 is up-regulated by glucose and by cholestyramine, and is down-regulated by chenodeoxycholic acid.
References
Product Information
FormatPurified
Control
  • Human liver tissue lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Cyp7a1 antibody is validated for use in WB & IHC for the detection of the Cyp7a1 protein.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImunnohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Cyp7a1 in rat and human liver tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Cyp7a1.
Clone15B9.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CYP7A1
  • CYP7
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~52 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cyp7a1 in 10 µg of human liver tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABD42 04053252854279

Documentation

Anti-Cyp7a1 Antibody, clone 15B9.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Cyp7a1 Antibody, clone 15B9.1 Certificates of Analysis

TitleLot Number
Anti-Cyp7a1, clone 15B9.1 2475858
Anti-Cyp7a1, clone 15B9.1 Monoclonal Antibody 2949965
Anti-Cyp7a1, clone 15B9.1 - Q2129760 Q2129760
Anti-Cyp7a1, clone 15B9.1 - 3173803 3173803
Anti-Cyp7a1, clone 15B9.1 - 3211121 3211121
Anti-Cyp7a1, clone 15B9.1 - 3328986 3328986
Anti-Cyp7a1, clone 15B9.1 - 3604327 3604327
Anti-Cyp7a1, clone 15B9.1 - 3978837 3978837
Anti-Cyp7a1, clone 15B9.1 - 4296841 4296841
Anti-Cyp7a1, clone 15B9.1 -2586831 2586831
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